硫普罗宁对小鼠实验性肝损伤的保护作用

Protection of tiopronin on experimental liver injury in mice

为观察硫普罗宁（ＭＰＧ）对小鼠实验性肝损伤的保护作用。取健康小鼠１２０只，随机分为４组，即溶剂对照组，模型对照组，ＭＰＧ高剂量组和ＭＰＧ低剂量组，每组３０只。采用腹腔注射给药，实验组（包括ＣＣｌ４及ＭＰＧ大小剂量组）先给体积分数为０２５％ＣＣｌ４１０ｍｌ／ｋｇ；对照组给等量溶剂。３０ｍｉｎ后，用ＭＰＧ７５及１５０ｍｇ／（ｋｇ·ｄ）进行治疗，连用１５ｄ；对照组给溶剂１０ｍｌ／ｋｇ。各组动物在实验的３６ｈ，７ｄ及１５ｄ分批处死，测定血清中丙氨酸氨基转换酶（ＡＬＴ），天门冬氨酸氨基转换酶（ＡＳＴ），丙二醛（ＭＤＡ）及病理组织学的改变情况。结果：ＭＰＧ剂量为７５及１５０ｍｇ／（ｋｇ·ｄ），血清中ＡＬＴ，ＡＳＴ及ＭＤＡ明显降低，肝细胞变性，坏死明显减轻。提示：ＭＰＧ对由ＣＣｌ４所致的急性肝损伤有明显的治疗作用。

Abstract The aim of this experiment was to obsererve the protective action of tiopronin(MPG) on experimental liver injury in mice. Kenming mice were used and divided at random into Four group: solvent control, model control, MPG h and MPG l groups, of 30 animals each. All the mice save mice from solvent group, were received of CCl 4(0.25%) at 10 ml/kg and 30 min later, MPG was given to mice from MPG h and MPG l groups at doses 150 and 75mg/(kg·d) respectively for 15 d. Determinations of alanine amino transferase (ALT), aspartate amino transferase(AST) and MDA and morphological examination were carried out in all the mice from 4 groups. The results showed that the serum levels of ALT, AST and MDA in MPG h and l groups markedly decreased and the degeneration and necrosis of liver cells were also significantly light compared with the model control group. The results of biochemical and morphological investigations suggest that MPG has a potently protective action in liver injury induced by CCl 4 which may be related to scavenging free redicals.