摘要
以GPV VP1-VP3非重叠序列重组原核表达多肽为检测抗原,建立了鉴别GPV感染抗体的Dot-ELISA方法,试验确定检测抗原包被浓度分别为700ng;兔抗鹅IgG-HRP-标记抗体的最适稀释度为1∶200;被检血清最佳稀释度为1∶400。检测GPV血清抗体的阳性率为100%;检测鸡抗GPV VP3禽痘重组病毒血清的阳性率为0%。
The method of Dot-ELISA for detecting GPV antibody was established with recombinant prokaryotic expressed peptide of VP1 -VP3 non- repeated nucleotide sequences of GPV eapsid protein in experiment. The coating concentration of Dot-ELISA detecting antigen was 700ng. The optimum dilution of rabbit anti-goose IgG-HRP antibody and detected serum were 1：200 and 1：400 respectively. The positive rate of detected GPV serum antibody was 100%, and the other one of the chicken anti-GPV-VP3 gene recombinant poutry poxvirus serum antibody was 0%.
出处
《中国生物工程杂志》
CAS
CSCD
北大核心
2009年第9期68-70,共3页
China Biotechnology
基金
内蒙古科技厅自然基金(200507010409)资助项目
