睡莲和菠菜光合膜光化学活性及多肽组分的比较

A Comparative Study on Photochemical Activity and PolypeptideCompositions of Photosynthetic Membranes fromNymphaea alba and Spinacia oleracea

比较分析了水生植物睡莲（ＮｙｍｐｈａｅａａｌｂａＬ．）及陆生植物菠菜（Ｓｐｉｎａｃｉａｏｌｅｒａｃｅａ）类囊体膜ＰＳＩ、ＰＳＩＩ电子传递活性、吸收光谱、室温荧光发射光谱等光化学特性及类囊体膜的多肽组分。结果显示：睡莲类囊体膜ＰＳＩ、ＰＳＩＩ电子传递活性相对较弱，分别为菠菜的６０２１％和７０８２％，其室温吸收光谱蓝紫光区域（４３０ｎｍ）吸收较弱，没有明显的吸收峰，红光区域的吸收光谱和菠菜相似；睡莲类囊体膜６８５ｎｍ处具较强的荧光发射峰。但５３０～７８０ｎｍ区域无发射肩峰。类囊体多肽组分和菠菜相比较存在明显的差别。

The electron transfer rates, room temperature absorption spectrum, fluoresence emission spectrum and polypeptide compositions were observed for thylakoid membrane from hydrophyte Nymphaea alba L and terrestrial plant Spinacia oleracea L. Some experimental results are as follows: (1)Nymphaea alba had a lower electron transfer activity than Spinacia oleracea. (2)Nymphaea alba had one fluorescence emission peak at 685 nm, and fluorescence emission peak at the 730～780 nm was absent(Fig. 1) (3)Nymphaea alba was less capable for light absorption at 400～500 nm than that of Spinacia oleracea(Fig. 2). (4)Nymphaea alba thylakoid membrane polypeptide components were distinct from that of the Spinacia oleracea (Fig. 3, 4) as shown by analysis of thylakoid membrane polypeptides using SDS-PAGE.