浙江秀山海泥水溶体系的抑菌作用研究

THE ANTIBACTERIA EFFECTS OF WATER-SOLUBLE PHASE OF SEA MUD

采用固体培养基体外抑菌法,在不同浓度,相同pH条件下,研究了采自浙江秀山海泥制备的不同浓度的海泥水溶体系对大肠杆菌、金黄色葡萄球菌、黑曲霉菌和白色念珠菌的抑制效果及其最低抑菌浓度,同时与目前公认较好的合成防腐剂对羟基苯甲酸甲酯、对羟基苯甲酸丙酯进行对比,考察了海泥水溶体系的抑菌动力学曲线。结果表明,浙江秀山滩涂海泥对各种指示菌都有一定程度的抑制作用,且抑制作用随浓度的增大而增强,海泥水溶体系对大肠杆菌、金黄色葡萄球菌、黑曲霉菌和白色念珠菌的最低抑菌浓度(MIC)值分别为0.75、1.00、0.50、2.00,对细菌的抑菌效果与对羟基苯甲酸丙酯相当,且优于对羟基苯甲酸甲酯,对霉菌的抑菌效果与两个阳性对照相当,对酵母菌的抑菌作用弱于对羟基苯甲酸丙酯和对羟基苯甲酸甲酯。抑菌动力学曲线显示接种72h内,含有海泥水溶体系的培养基中的供试菌生长均被抑制在一个较低水平,达到100%的抑制。因此将其用于食品防腐、化妆品工业、医学领域具有广阔的前景。

Sea muds taken off Xiushan, Zhejiang were studied for examining in vitro inhibition effect on Escherichia coli, St. aureus, Aspergillus niger and Candida albicansa. The muds in different concentrations at the same pH value were sampled in different places. With well-accepted synthesized antiseptics on hydroxymethyl benzoate and propyl p-hydroxybenzoate, bacteriostasis kinetic curve of soluble phase of sea mud system was established. The results show that the sea muds show a similar antibacterial effect without significant difference （P〉0.05）. They inhibited bacteria to some degrees, which increased with the increase in sea mud concentration. The minimum inhibition concentration （MIC） of a sea mud on Escherichia coli, St. aureus, Aspergillus niger, and Candida albicansa is 0.75, 1.00, 0.50, and 2.00, respectively. Compared with hydrobenzoate, the inhibition effect of water-soluble phase is similar to that of propyl p-hydroxybenzoate but better. Compared with hydroxymethyl benzoate and propyl p-hydroxybenzoate, the inhibition effect on mold is similar; on yeast is weaker. Bacteriostasis kinetic curve shows that a sea mud containing medium （within 72h after inoculation） depressed the growth of test strains to a lower level in 100% coverage. Therefore, antibacterial application of sea mud shall be promising in food preservation, cosmetic industry and medical field.