西瓜甜瓜质量性状的分子标记与定位研究进展

Progress in Research on Molecular Markers and Mapping of Quality Traits in Watermelon(Citrullus lanatus) and Melon(Cucumis Melo L.)

分子标记在作物育种、资源遗传多样性分析、遗传图谱构建等方面有着广泛的应用,常见的分子标记方法主要有RFLP、RAPD、SSR、AFLP、SCAR、CAPS、SNP等。迄今为止,国内外学者已经利用分子标记技术对西瓜和甜瓜的20多个质量性状进行了标记和定位。本文主要对西瓜和甜瓜质量性状的分子标记和定位研究进展进行了综述,发现RAPD标记使用最多,而更具优势的SSR标记使用较少;对抗病虫等育种相关的基因研究较多,且多数标记遗传距离偏大,达不到图位克隆的要求,而对西瓜和甜瓜遗传基础相关的基因研究较少。

Molecular markers have a wide range of applications such as marker-assisted selection, analysis of genetic diversity of germplasm resoures, constrcution of genetic linkage maps ect. RFLP, RAPD, SSR, AFLP, SCAR, CAPS, SNP are common methods of molecular markers. So far,domestic and foreign researchers have used molecular markers to marker and mapping more than 20 quality traits in watermelon and melon. Among them the RAPD molecular marker is the method that used most widely, while the advantage of SSR marker is used less than other markers. The current molecular markers major research on plant diseases and insects resistance genes, and most of the linkage markers have a lager genetic distance to the target genes, which not suitable for map-based cloing. But the basic of genetics genes have not sufficiently research. This article summarized the molecular markers and mapping of quality traits research progress in watermelon and melon.