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虎杖苷对CCl_4损伤原代培养大鼠肝细胞的保护作用 被引量:47

Protective effects of polydatin on CCl 4 induced injury of primary cultured rat hepatocytec
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摘要 目的研究虎杖苷(PD)对CCl4损伤原代培养大鼠肝细胞的保护作用。方法原位灌流法分离大鼠肝细胞,培养24h后加入PD,同时造成CCl4损伤,分别于损伤后6,12,24和48h测培养液中谷丙转氨酶(ALT)、丙二醛(MDA)和还原型谷胱甘肽(GSH)活性,48h后用MTT比色法测肝细胞存活率。结果PD1×10-7~1×10-4mol·L-1能不同程度地抑制ALT和MDA的上升,提高GSH水平,显著提高细胞存活率,以1×10-5mol·L-1效果最好。 AIM To investigate the effects of polydatin (PD) on CCl 4 intoxicated primary cultured rat hepatocytes. METHODS Rat hepatocytes were separated by perfusion with collagenase solution. PD was added into the culture medium after incubating the rat hepatocytes for 24 h, At the same time 10 mol·L -1 CCl 4 was added to induce injury. The level of ALT, malondialdehyde(MDA), reduced glutathione (GSH) and cell viability after cultivation for 6, 12, 24 and 48 h were determined respectively. RESULTS PD 1×10 -7 ~1×10 -4 mol·L -1 significantly improved the number of cell viability and prevented the decrease of GSH, ALT and MDA levels. PD 1×10 -5 mol·L -1 showed the most effect. CONCLUSION PD 1×10 -7 ~1×10 -4 mol·L -1 could protect primary cultured rat hepatocytes against CCl 4 induced injury.
出处 《中国药理学通报》 CAS CSCD 北大核心 1998年第6期543-545,共3页 Chinese Pharmacological Bulletin
基金 国家中医药管理局资助
关键词 虎杖苷 大鼠 肝细胞 CCl4损伤 保护作用 polydatin rat hepatocytes CCl 4 intoxicate
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