RKIP在胆囊癌中的表达及临床意义

Expression of RKIP and its clinical significance in gallbladder carcinoma

目的:探讨胆囊癌中Raf激酶抑制蛋白(RKIP)的表达状况及其与胆囊癌临床病理指标和预后的可能关系.方法:2006-01/2009-06苏州大学附属第二医院胆囊癌患者50例,外科手术切除术后病理证实的存档组织蜡块.所有病例术前未进行放化疗,有完整的临床病理资料.采用免疫组织化学ElivisionPlus法,对50例原发性胆囊癌与38例慢性胆囊炎组织中RKIP表达进行检测.结果:在胆囊癌组织中表达的阳性率明显高于慢性胆囊炎组织,差异有统计学意义(74.0%vs0.8%,P<0.01).RKIP表达水平与胆囊癌患者年龄、性别、组织学类型及是否伴有结石等因素均无明显关系(P>0.05),但RKIP高表达与胆囊癌的低分级(31.8%vs92.3%,P<0.05)、淋巴结或远处转移(75.0%vs100.0%,P<0.05)以及术后生存时间减少(50.0%vs93.8%,P<0.05)有明显关系.结论:RKIP在胆囊癌中高表达可能提示其在胆囊癌的发生、发展中起重要作用.

AIM： To investigate the expression of Raf kinase inhibitory protein （RKIP） in gallbladder carcinoma and to explore its possible relationship with clinicopathological parameters and prognosis of gallbladder carcinoma. METHODS： RKIP expression was examined by streptavidin peroxidase immunohistochemical method on paraffin-embedded tissue specimens from 50 patients with gallbladder cancer and 38 with chronic cholecystitis who underwent surgical treatment between January 2006 and June 2009 in Suzhou University. None of these patients received preoperative treatment, and detailed clinicopathological information was provided. RESULTS： The positive expression rate of RKIP in gallbladder carcinoma was significantly higher than in chronic cholecystitis, with statistically significant difference （74.0% vs 0.8%, P 〈 0.01）. In gallbladder carcinoma, the expression of RKIP had no correlation with age, gender, histologic type and gallbladder stone （P 〉 0.05）, but over- expression of RKIP correlated significantly with lower histological grading （31.8% vs 92.3%, P 〈 0.05）, lymph node or distant metastasis （75.0% vs 100.0%, P 〈 0.05）, and shorter survival after operation （50.0% vs 93.8%, P 〈 0.05）. CONCLUSION： RKIP is overexpressed in de- velopment of gallbladder carcinoma. RKIP may play an important role in tumor progression of human gallbladder carcinoma.