基于分子印迹技术的丙溴磷压电石英晶体微天平研制

Development on quartz crystal microbalance for the determination of profenofos using molecularly imprinted polymers as recognition element

介绍了一种用于检测丙溴磷农药的分子印迹压电生物传感器的构建方法。采用沉淀聚合法合成了农药丙溴磷的分子印迹聚合物,将其固定于石英晶体微天平电极表面构建传感器;采用环境扫描电镜以及原子力显微镜对聚合物形貌、传感器电极表面形貌特征进行分析,并利用传感器对丙溴磷农药进行检测分析,其质量浓度在10-1000 ng/mL范围内,传感器频率改变与丙溴磷浓度之间的响应呈线性关系,线性方程为y=0.139ρ＋2.26（r=0.9984）。结果表明,构建的分子印迹压电生物传感器能够对农药进行初步检测,具有较高的灵敏性和较好的特异识别能力。

A new quartz crystal sensor using molecularly imprinted polymers （MIPs） as recognition element has been prepared for the fast determination of profenofos. Firstly, the molecularly imprinted polymers were prepared by precipitation polymerization, and then the polymer particles were fixed on the serface of the electrode. Scanning electron microscopy （SEM） and atomic force microscopy （AFM） were employed to evaluate the obtained imprinted polymer particles and the MIP sensitive film coated on the electrode. The sensor exhibits a linear relationship between the frequency shift and profenofos concentration in the range of 10 ng/mL to 1000 ng/mL （y = 0. 139ρ ＋ 2.26, r = 0.9984）. The experiments indicate that the obtained sensor has high sensitivity, excellent selectivity and good reproducibility.