流式细胞术检测胃癌前病变DNA及p21,p53含量

Detection of DNA and Content of p21 and p53 for Gastric Precancer Lesion by Flow Cytometry

应用流式细胞术和免疫荧光技术，对８０例胃癌前病变细胞中ＤＮＡ含量，ｒａｓｐ２１和ｐ５３蛋白进行定量检测，探讨其在胃癌前病变中，作为癌变早期标志物的临床意义．检测结果发现，胃癌前病变的ＤＮＡ异倍体率随不典型增生病变的分级增高而增加，ｒａｓｐ２１和ｐ５３蛋白的表达量亦随胃癌前病变不典型增生程度的加重而增高．ＤＮＡ异倍体和ｒａｓｐ２１表达阳性的胃癌前病变出现的癌变率显著增高，提示可能是癌变早期的分子标志物．

Content of DNA, rasp21 and p53 in 80 case gastric precancer lesion was quantitative detected by flow cytometry and immunofluorescence techniques. and to investigate its in clinical value as early carceration marker.The detected results showed that raising in DNA heteroploid rate. Rasp21 and p53 protein expression were found with raising of dysplasia grade in gastric precancer lesion. The rate of canceration of gastric precancer lesionin in DNA heteroploid and rasp21 expression positive were notable increased. DNA heteroploid and rasp21 protein overexpression can be an early canceration molecular marker.