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维生素D_3联合高脂饲料建立大鼠动脉粥样硬化模型 被引量:47

Establishment of a rat model of atherosclerosis using vitamin D_3 and high-fat diet
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摘要 目的:探索维生素D3(VD3)联合高脂饲料快速建立大鼠动脉粥样硬化模型的方法。方法:健康雄性Wistar大鼠60只,分为对照组、高脂组、模型组。4周后,检测血清中Ca2+、TG、TC、HDL-C、LDL-C的含量,制作胸主动及肝脏切片进行形态学观察。结果:模型组与对照组和高脂组相比,各血清学指标均明显升高(P﹤0.05);模型组胸主动脉内膜下可见明显的钙化、平滑肌细胞增生及泡沫样细胞,而高脂组大鼠胸主动脉未见明显变化;模型组肝细胞内有大量的脂滴空泡,肝系数明显高于高脂组和对照组(P﹤0.05)。结论:大鼠腹腔注射VD370万U/kg,联合高脂饲料喂养,可快速成功复制大鼠动脉粥样硬化模型。 Objective To explore an efficient method to establish a rat model of atherosclerosis using vitamin D3 and high-fat diet. Methods Sixty healthy Wistar rats were randomly divided into 3 groups: control group, high-fat diet group and model group. Four weeks later, calciumion, triglyeride, total cholesterol, high-density lipoprotein and lowdensity lipoprotein in rat serum were detected, and the sections of thoracic aorta and liver tissue were prepared for morphology observation. Results The levels of serologic indexes in model group were obviously higher than those in control group and in high-fat diet group(P 〈 0.05); Obvious calcification, smooth muscle cell proliferation and foam cells could be observed under aortic intima in model group. Lots of fat drop vacuoles were appeared in the liver cells in the model group, and the liver coefficient in model group was obviously higher than that in control group and in high-fat diet group(P 〈 0.05). Conclusion The rat model of atherosclerosis can be successfully established using vitamin D3 combined with high-fat diet.
出处 《实用医学杂志》 CAS 北大核心 2009年第21期3569-3571,共3页 The Journal of Practical Medicine
基金 国家教育部留学归国人员启动基金资助课题(编号:1999-363) 吉林大学创新基金资助课题(2002)
关键词 动脉粥样硬化 VD3 模型 大鼠 Atherosclerosis Vitamin D3 Model Rat
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