文冠果细胞悬浮培养技术研究

Study on Cell Suspension Culture Technology of Xanthoceras sorbifolia

[目的]建立文冠果体细胞胚胎发生和快速成苗的悬浮培养体系,为文冠果的产业化和规模化发展提供参考。[方法]以文冠果新旧种子为材料,诱导体胚发生,进行文冠果细胞悬浮培养。[结果]适合胚性愈伤诱导的最佳培养基为MS+6-BA1.0mg/L+NAA0.5mg/L+2,4-D0.5mg/L+3%蔗糖;适合胚性愈伤组织不定芽诱导培养的最佳诱导培养基为MS+6-BA1.0mg/L+NAA1.0mg/L+3%蔗糖,且萌芽率最高;适合单细胞团再生植株培养的最佳培养基为MS+6-BA0.5mg/L+NAA0.2mg/L+3%蔗糖。[结论]NAA和2,4-D混合使用有利于胚性愈伤组织的形成;一定浓度的NAA对文冠果愈伤组织萌芽培养有较大的促进作用。

[ Objective ] To establish suspension culture system of cell embryogenesis and fast seedling formation of Xanthoceras sorbifolia, and provide reference for the developments of industrialization and scale. [ Method ] Taking new and old seed of Xanthoceras sorbifolia as material, inducing embryogenesis, the cell suspension culture of Xanthoceras sorbifolia was carried out. [ Result] The optimal medium of MS ＋ 6-BA 1.0 mg/L ＋ NAA 0.5 mg/L ＋ 2,4-D 0.5 mg/L ＋ 3 % sucrose was suitable for embryogenic callus induction. The optimal medium of MS ＋ 6-BA 1.0 mg/L ＋ NAA 1.0 mg/L ＋ 3% sucrose was suitable for adventitious buds induction culture of embryogenie callus, germination rate was highest. The optima] medium of MS ＋ 6-BA 0.5 mg/L ＋ NAA 0.2 mg/L ＋ 3% sucrose was suitable for regenerated plant culture of single cell mass. [ Conclusion] NAA mixed with 2,4-D was more beneficial to the formation of embryogenie callus. NAA has large promoting effect on callus germination culture of Xunthoceras sorbifolia at some concentration.