破裂性椎间盘突出重吸收机制的研究

A Rat Model of Disc Herniation Spontaneous Resorption

目的探讨破裂性椎间盘突出发生重吸收的作用机制。方法将20只远交群雄性大鼠随机分成对照组、实验组。实验组经手术切除尾椎椎间盘,埋植在硬膜外。30d后处死,取埋植髓核组织,进行HE染色及免疫组化检测。对照组将线团埋植在背部肌肉内,30d后处死作试验对照。结果与对照组比较实验组埋植组织的质量明显缩小,实验组的TNF-ɑ、VEGF免疫组化染色呈阳性。结论椎间盘突出后发生的重吸收与其游离接触血运有关,新生血管与炎性反应在重吸收过程中发挥很大作用。

Objective To investigate the histopathological changes of sequestration-type herniated disc, and the related factors of its absorption. Methods Twenty male Sprague-dawley （SD） rats were chosen.The rats were divided into control group and experimental group at random. In the experimental group, discs were harvested from the caudal vertebrae and placed subcutaneously in the epidural. After 30 days,the rats were killed and discs were harvested. HE staining and immunohistochemistry were used for analysis of cells and tissue. In the control group, coil was placed subcutaneously in the epidural.Results Compared with the control group, quality and shape displayed sig- nificant decrease in the experimental group.The TNF-α and VEGF pulposus were observed in experimental group with immunohistochemisty.Conclusion The extent of spontaneous absorption mainly depends on the origin of the sequestration tissue.The VEGF and TNF-α play important roles in the absorption process.