生长因子在视网膜增殖膜的表达

EXPRESSION OF GROWTH FACTORS IN MEMBRANES OF PROLIFERATIVE RETINOPATHY

目的：了解血小板源性生长因子（ＰＤＧＦ）和碱性成纤维细胞生长因子（ｂＦＧＦ）在视网膜增殖膜细胞的表达及其与增殖性玻璃体视网膜病变（ＰＶＲ）的关系。方法：免疫组化法研究ＰＤＧＦ和ｂＦＧＦ在２０例视网膜前膜和１例视网膜下膜（视网膜脱离术后继发）细胞中的表达情况。结果：①１５／２１（７１．４％）例标本对抗ＰＤＧＦ抗体呈阳性反应，１４／２１（６６．７％）例对抗ｂＦＧＦ呈阳性反应；②Ｅａｌｅｓ病和增殖性糖尿病视网膜病引起的血管增殖性前膜（≥６１个细胞／视野，１０００×）较ＰＶＲ引起的非血管增殖性前膜（３１～６０个细胞／视野）内细胞密度高，黄斑前膜（≤３０个细胞／视野）最低。结论：ＰＤＧＦ和ｂＦＧＦ在ＰＶＲ发展过程中起重要作用；ＰＶＲ膜上增殖细胞可产生ｂＦＧＦ，它是导致严重ＰＶＲ时玻璃体内ｂＦＧＦ含量异常升高的主要原因。

Objective: To study the role of platelet derived growth factor (PDGF) and basic fibroblast growth factor (bFGF) which play in PVR. Methods: Using the immunohistochemistry technique to study the expression of PDGF and bFGF in 20 epiretinal membranes and 1 subretinal membrane. Results: ①In 21 pieces, expressed PDGF in 15/21 (71 4%); expressed bFGF in 14/21 (66 7%); ②The cellular density was different from the different type of epiretinal membranes. The cellular density of vascular membrane induced by PDR and Eales′ disease was the highest (≥61 cells/field, 1 000×); the nonvascular membranes induced by PVR or traumatic PVR were middle (31～60 cells/field); and the macular membrane was the lowest (≤30 cells/field). Conclusion: The PVR cells synthese and secrete PDGF and bFGF, implicate that PDGF and bFGF play an important role in PVR; and prove that the increase of bFGF in PVR vitreous were caused by PVR cells locally.