冻结肩患者关节囊组织中TGF-β和MMPs表达研究

Expression of transforming growth factor-β and metalloproteinases in joint capsule of frozen shoulder

目的研究转化生长因子-β(TGF-β)和基质金属蛋白酶(MMPs)在冻结肩发生中的可能作用。方法以12例接受肩关节镜手术的冻结肩患者为研究对象,术中取肩袖间隙部位关节囊组织,Real-time PCR和Western blotting分别检测TFG-β、MMP1、MMP2、MMP3、MMP9、MMP12 mRNA和蛋白表达。以同期接受肩关节镜手术的12例非冻结肩患者作为对照,其中肩关节不稳2例、肩袖损伤5例、肩峰下撞击症5例。结果冻结肩组和对照组关节囊组织TGF-β mRNA表达分别为3.36×104±2.18×103和1.85×104±3.31×103,蛋白表达分别为1.55±0.33和1.13±0.21,两组比较差异均有统计学意义(P<0.05)。冻结肩组关节囊组织MMP1、MMP2、MMP3、MMP9、MMP12 mRNA和蛋白表达均显著高于对照组(P<0.05)。结论冻结肩的发生可能与患者关节囊组织中TFG-β和MMPs的高表达有关。

Objective To explore the role of transforming growth factor （TFG）-β and matrix metalloproteinases（MMPs）in the development of frozen shoulder. Methods Twenty-four patients who underwent shoulder arthroscopy were included,and were divided into frozen shoulder group （ n =12） and control group （ n =12; n =2 for shoulder instability,n =5 for rotator cuff tear and n =5 for subacromial impingement）. Joint capsule tissues at the rotator cuff interval were obtained,and the expression of TGF-β,MMP-1,MMP-2,MMP-3,MMP-9 and MMP-12 mRNA and protein was detected by Real-time PCR and Western blotting,respectively. Results The expression of TGF-β mRNA in frozen shoulder group and control group was 3.36×10 4±2.18×10 3 and 1.85×10 4±3.31×10 3,respectively,the expression of TGF-β protein was 1.55±0.33 and 1.13±0.21,respectively,and there were significant differences between these two groups （ P 0.05）. The expression of MMP1,MMP2,MMP3,MMP9 and MMP12 mRNA and protein in frozen shoulder group was significantly higher than that in control group（ P 0.05）. Conclusion The higher expression of TGF-β and MMPs in joint capsule of frozen shoulder may be associated with the development of frozen shoulder.