人参多糖对冷应激大鼠颗粒细胞与黄体细胞某些功能的影响

Effects of Ginseng Polysaccharose on Functions of Rat Granulose and Luteal Cells under Cold Stress in vitro

目的研究人参多糖对冷应激雌性大鼠卵巢黄体细胞与颗粒细胞的作用。方法分离培养大鼠黄体细胞与颗粒细胞,按加药因素分组,以加人参多糖为实验组,分为黄体细胞实验组、颗粒细胞实验组;以不加人参多糖为对照组,分为黄体细胞对照组、颗粒细胞对照组。分别采用37、4、0℃进行冷应激实验。采用放免分析方法测定孕酮与cAMP的含量。结果37℃时,黄体细胞实验组孕酮含量为(5291.46±185.14)pmol,低于黄体细胞对照组(6573.55±326.82)pmol,颗粒细胞实验组孕酮含量为(153.16±42.87)pmol低于颗粒细胞对照组(197.42±92.46)pmol,差异均有统计学意义(P<0.05)。0℃时,黄体细胞对照组孕酮含量为(5077.37±312.47)pmol、颗粒细胞对照组孕酮含量为(149.24±25.61)pmol,分别低于37℃时黄体细胞、颗粒细胞对照组孕酮含量,差异有统计学意义(P<0.05)。黄体细胞实验组cAMP含量在37、4、0℃分别为(65.26±15.93)、(56.39±12.95)、(39.60±14.69)fmol,分别高于37、4、0℃黄体细胞对照组(31.20±17.13)、(32.76±12.88)、(20.84±13.19)fmol,颗粒细胞实验组cAMP含量在37、4、0℃分别为(296.42±27.28)、(250.69±22.29)、(239.97±17.03)fmol,分别高于37、4、0℃颗粒细胞对照组(121.15±19.96)、(189.63±10.88)、(106.10±14.05)fmol,差异均有统计学意义(P<0.05)。结论冷应激使孕马血清和人绒毛膜促性腺激素(HCG)诱导的卵巢黄体细胞与颗粒细胞孕酮和cAMP含量降低,人参多糖可抑制孕酮分泌。

Objective To study the effects of ginseng polysaccharose on functions of rat luteal and granulose cells under cold stress in vitro. Methods Rat luteal and granulose cells were cultivated and separated. Luteal and granulose cells were taken as the control group, and the groups added with ginseng polysaccharose were taken as the experiment groups if grouped by the addition of ginseng polysaccharose. If grouped by the cold stress factor, the group of 37℃ was taken as the control group, and the groups of 4℃ and 0℃ were taken as the experiment groups. The contents of progesterone and cyclic AMP （cAMP） were determined by radioimmunoassay kit （RIA kit）. Results At 37℃, progesterone content in luteal cell of ginseng polysaccharose experiment group was（5291.46±185.14）pmol which was lower than that of luteal cellcontrol group without ginseng polysaccharose（6573.55 ±326.82）pmol. Progesterone content in granulose cell of ginseng polysaccharose experiment group was（153.16 ±42.87）pmol which was lower than that of granulose cell control group without ginseng polysaccharose（ 197.42± 92.46） pmol. The differences were significant （P 〈0.05）. At 0℃, progesterone content in luteal cell in cold stress experiment group without ginseng polysaccharose was（5077.37±312.47）pmol, and progesterone content in granulose cell was（149.24±25.61） pmol. Both of them were lower than those of the 3722 luteal cell and granulose cell control groups without ginseng polysaccharose respectively （P 〈 0.05）. The luteal cell cAMP contents of ginseng polysaccharose experiment groups at 3722, 4℃ and 022 were （65.26 ± 15.93 ）, （ 56.39 ± 12.95 ） and （39.60 ± 14.69 ） fmol respectively, and all of them were higher than those of 3722 , 4℃ and 0℃ control groups without ginseng polysaccharose（31.20± 17.13）, （32.76±12.88）and（20.84±13.19）fmol. The cAMP contents in granulose cell of ginseng polysaccharose experiment groups at 3722, 422 and 022 were（296.42± 27.28）, （250. 69 ± 22.29）and（239.97±17.03）fmol respectively, and all of them were higher than those of 37℃, 422 and 0℃ control groups without ginseng polysaccharose（ 121.15± 19.96 ）, （ 189.63 ± 10.88） and（ 106. 10± 14.05 ） fmol respectively（P〈 0. 05）. Conclusions Results demonstrate that cold stress could reduce progesterone and cAMP contents of rat luteal and granulose cells induced by pregnant mare serum and human chorionic gonadotropin（HCG）, and ginseng polysaccharose might inhibit the secretion of progesterone.