芍药苷对四氯化碳诱发肝纤维化大鼠血清TGFβ1 TMP1 MMP2及MMP9的影响

Effects of Paeoniflorin on Serum Levels of TGFβ1 TMP1 MMP2 MMP9 in Hepatic Fibrosis Induced by Carbon Tetrachloride in Rats

目的:探讨芍药苷对四氯化碳诱发肝纤维化大鼠血清TGFβ1、TMP1、MMP2及MMP9的影响。方法:清洁级Sprague-Dawley大鼠60只,随机分成6组,A组(正常对照组)、B组(模型组)、C1组(芍药苷小剂量组)、C2组(芍药苷中剂量组)、C3组(芍药苷大剂量组)、D组(水飞蓟素对照组)。除正常对照组外,均经皮下注射400mL/L四氯化碳8周,后各治疗组给予不同剂量芍药苷干预8周,阳性对照组给予水飞蓟素干预8周,ELISA法检测大鼠血清TGFβ1、MMP2、MMP9及TMP1。结果:TGFβ1:与B组比较,A组P<0.01,C1组、C2组、C3组、D组P<0.05;TMP1:与B组比较,A组P<0.01,C1组、C2组、C3组、D组P<0.05;MMP2:与B组比较,A组P<0.01,C1组、C2组、C3组、D组P<0.05;MMP9:与B组比较,A组P<0.01,C1组、C2组、C3组、D组P<0.05;结论:芍药苷能够通过降低大鼠血清TGFβ1、TMP1、提高大鼠血清MMP2、MMP9而发挥抗肝纤维化作用,并且量效关系明显。

Objective：To investigate the effects of paeoniflorin on serum levels of TGF β 1, TMP1, MMP2, MMP9 in hepatic fibrosis induced by carbon tetrachloride in rats. Methods： Sixty Sprague-Dawley rats were divided into 6 groups, namely normal control group （A）, model group （B）, paeoniflorin low （C1）, medium （C2）, high （C3） dose group and silymarin contrast group （D）. The rats of B, C1, C2, C3 and D were subcutaneously injected by carbon tetrachloride （3mL/kg body weight ） twice a week for 8 weeks.The rats of C1, C2, C3 were then administered with paeoniflorin at the doses of 5g/kg body weight （C3）, 2.5g/kg （C2）, 1.2g/kg （C1） for 8 weeks. The rats of D were then administered with silymarin at a dose of 50mg/kg body weight. The changes of serum levels of TGF β 1, TMP1, MMP2, MMP9 were measured by elisa. Results： As comparied with those in rats of group B, the serum levels of TGF β 1 （group A P〈0.01, group C1, group C2, group C3, group D both P〈0.05）, TMP1 （group A P〈0.01, group C1, group C2, group C3, group D both P〈0.05）, were significantly decreased in group A, C 1, C2, C3, and D. As comparied with those in rats of group B, the serum levels of MMP2 （group A P〈0.01, group C1, group C2, group C3, group D both P〈0.05）, MMP9 （group A P〈0.01, group C1, group C2, group C3, group D both P〈0.05）, were significantly increased in group A, C1, C2, C3, and D.Conclusion：Paeoniflorin was able to reduce hepatic fibrosis induced by carbon tetrachloride in rats and its mechanisms maybe the changes of TGF β 1, TMP1, MMP2, MMP9.