期刊文献+

10株水痘带状疱疹病毒分离鉴定与生物学特性研究 被引量:1

Study of the Isolation and Identification and Biological Property of Ten-Strain Variclla-Zoster Viruses
下载PDF
导出
摘要 用Vero-E6细胞从12例水痘及带状疱疹病人水疱液中分离到10株病毒,分离阳性率为83.3%。10株病毒均可使感染细胞圆缩融合、脱落等典型的VZV局灶性细胞病变(CPE)特点,CPE随着传代次数增加而加快。用带状疱疹恢复期病人血清作间接免疫荧光染色镜检可见典型的感染细胞核内荧光块。10株病毒感染细胞制成的抗原片检测5例带状疱疹病人急性期及恢复期血清荧光抗体滴度均有4倍以上增高。VZV对Vero细胞敏感;对沙鼠肾,胎兔肾,胎豚鼠肾、肺、脾原代单层细胞不敏感;对乳小白鼠不敏感。毒种在-20℃保存一周内死亡,但在30%脱脂牛奶,20%小牛血清及10%山梨醇Eagle′s液中,液体或真空冰冻干燥-100℃可保存二年以上。 Itakurs N,Hiroishi 10 Strains viruses were isolated from vesicle fluid of skin Iesions of 12 patients with Variclla or herpes zoster by means of Vero-E6 cells.The positive rate was 83.3 percent.These Viruses all cause typical cell damages such as apoptosis,fusion and fallout.These damages got quick following increased future degenerations.The typical fluorescence lump of nucleus within the infected cells would be found by indirect immunofluorescence technique.The antigen samples prepared with virus-infecting cells could be used to examine the serums of patients with herpes zoster and it could be found that the antibody titer in recovering stage was four times as much as that in acute stage.VZV was sensitive to Vero cells and not sensitive to the primary simple-lager cells from Merioney Unguiculatus kidney,fetal rabbit kidney and fetal guinea pig kidney,lung and spleen.VZV was insensitive to newborn white mouse too.The viruses would die within one week at-20℃,but they can be preserved in Eagle's solution containing de-fat milk,newborn bovine serum and sorbitol at -100℃over two year.
出处 《中国卫生检验杂志》 CAS 1998年第4期197-201,共5页 Chinese Journal of Health Laboratory Technology
关键词 小痘 带状疱疹病素 分离 鉴定 Variclla-Zoster Viruses Isolation Biological Property.
  • 引文网络
  • 相关文献

参考文献3

共引文献1

同被引文献7

引证文献1

二级引证文献68

;
使用帮助 返回顶部