摘要
目的:研究雌激素受体β(estrogen receptorβ,ERβ)对人牙周膜成纤维细胞(human periodontal ligament,HPLF)核因子-κB受体活化因子配体(receptor activator of NF-κB ligand,RANKL)表达的影响。方法:采用基因干涉方法抑制了HPLF细胞中ERβ基因的表达,用雌激素干预ERβ抑制的和未抑制的HPLF细胞,研究细胞RANKL表达的情况。结果:雌激素明显降低了ERβ抑制的HPLF细胞RANKL的表达,但对ERβ未抑制的HPLF细胞RANKL的表达没有显著影响。结论:雌激素可能通过ERβ抑制牙周膜成纤维细胞RANKL的表达。
Objective: To investigate the effect of ERβ on the expression of RANKL in human periodontal ligament (HPLF)cells. Methods: The short interfering RNA (siRNA) technique was employed to inhibit ERβ expression in HPLF cells and cells were cultured with saturating 17β-estradiol (10^-7 mol/L) for 48 hours. Changes of the ex pression of RANKL were determined by RT-PCR and Western blot analysis. Results: It was shown that estradiol caused decrease of RANKL expression in HPLF ceils. However, estradiol had no effect on the expression of RANKL in HPLF - siERβ cells. Conclusion: These results indicate that ERβ is of major importance for estrogenmediated effects on gene transcription in HPLF cells and estrogen influences the expression of RANKL in HPLF ceils via ERβ.
出处
《口腔医学研究》
CAS
CSCD
北大核心
2009年第6期684-687,共4页
Journal of Oral Science Research
基金
国家自然科学基金(编号:30572069)