静注免疫球蛋白对新生儿单个核细胞IL-2、IL-4 mRNA表达的调节

The regulatory effects of intravenous immunoglobulin on the expression of IL 2 and IL 4 mRNA in neonatal mononuclear cell

目的探讨静注免疫球蛋白（ＩＶＩＧ）抑制体外脐血Ｂ细胞免疫球蛋白释放的机制。方法运用ＲＴ－ＰＣＲ法观察ＩＶＩＧ对体外新生儿单个核细胞（ＣＢＭＣ）分泌的ＩＬ－２ｍＲＮＡ及ＩＬ－４ｍＲＮＡ表达的影响。结果佛波醇乙酯结合钙离子导入剂（ＰＭＡ＋ＩＯＮＯ）激活ＣＢＭＣ，很容易检测到ＩＬ－２ｍＲ－ＮＡ的表达，但不能检测出ＩＬ－４ｍＲＮＡ的表达。将ＰＨＡ刺激后的ＣＢＭＣ与ｒＩＬ－２一起孵育，再用ＰＭＡ＋ＩＯＮＯ刺激，ＣＢＭＣ始表达ＩＬ－４ｍＲＮＡ。在活化后的ＣＢＭＣ接受ＰＭＡ＋ＩＯＮＯ再刺激的同时加入ＩＶＩＧ（５ｍｇ／ｍｌ）。发现ＩＶＩＧ抑制了上述两种细胞因子ｍＲＮＡ的表达。结论ＩＶＩＧ对ＩＬ－２。

Objective To study the mechanisms of inhibitory effect of intravenous immunoglobulin(IVIG) on immunoglobulin production of cord blood B cell in vitro. Methods With the method of RT PCR,the expressions of IL 2 and IL 4 mRNA secreted by neonatal mononuclear cell(CBMC) were investigated. Results It was easy to detect the expression of IL 2 mRNA but not that of IL 4 mRNA in primed CBMC stimulated by PMA combining ionomycin.The PHA stimulated CBMC which was then incubated with rIL 2 began to express IL 4 mRNA after restimulation of PMA plus iono.Addition of IVIG (5mg/ml) to the primed CBMC which was restimulated with PMA plus ionomycin resulted in a significant inhibition of the expression of IL 2 and IL 4 mRNA. Conclusions The IVIG mediated decreased secretion of IL 2 and IL 4 may be due to the inhibitory effect of IVIG exerted on any phase of the entire course from transcription to secreting into extracellular tissues.