乙型肝炎患者外周血单个核细胞IL─4、IFN─γ水平的检测及其意义

Detetion and significance of IL-4 and IFN-γ levels in PBMCs of patients the chronic hepatitis B

目的了解白细胞介素─4（IL─4）和γ─干扰素（IFN─γ）与乙型肝炎（乙肝）发病机理关系并探讨其临床意义。方法检测69例不同临床类型的慢性乙肝患者的外周血单个核细胞（PBMCs）在体外经重组乙肝e抗原（rHBeAg）和重组乙肝核心抗原（rHBcAg）诱导后产生的IL─γ和IFN─γ水平。结果慢性重度组，慢性重型组IFN─γ水平明显高于对照组（P＜0．05～0．01）及慢性轻度组乙肝患者（P＜0．05）；rHBeAg、rHBcAg诱导后各组间IL─4水平无明显差异（P＞0．05）。结论IL─4和IFN─γ在不同临床类型乙肝的发病及病情发展中起着各自的作用。

Objective To explore the relationship between IFN-γ, IL-4 levels and hepatitis B pathogenesis.Method After peripheral blood mononuclear cells (PBMCs) of 69 patients with chronic hepatitis B had been stimulatedwith rHBeAg and rHBcAg, the levels of IL-4 and IFN-γ were measured by EIJSA. Those patients were divided intofive groups accoding to their clinical types. Results The level Of IFN-γ in chronic severe and chronic severe-degree hepatitis B groups was higher than that in control (P<0.05~0.01) and mild-degree group (P<0.05). IL-4level had no significant difference among five groups. Conclusion IL-4 and IFN-γ played own important roles in chronic hepatitis B Pathogenesis and development.