莫诺苷对大鼠局灶性脑缺血再灌注损伤神经功能的影响

Morroniside Ameliorate Focal Cerebral Ischaemia-reperfusion Injury Induced Neurological Function Deficits in Rats

目的观察中药山茱萸的有效成分莫诺苷对大鼠局灶性脑缺血再灌注后神经功能的影响。方法Wistar雄性大鼠随机分为假手术组、模型组、莫诺苷小剂量组(30 mg/kg)、莫诺苷中剂量组(90 mg/kg)、莫诺苷大剂量组(270 mg/kg)、维生素E(VE)(35 mg/kg),采用线栓法制作大鼠局灶性脑缺血再灌注模型,缺血30 min后再灌注3 d,应用Zea Longa法、爬网格、平行木、吊绳、Ludmila Belayev 12分评分法,观察莫诺苷对神经功能缺损的改善作用。结果与模型组比较,莫诺苷给药组(小、中、大剂量)Zea Longa法评分均差异极显著(P<0.01);与模型组比较,莫诺苷给药组(小、中、大剂量)吊绳法评分均差异极显著(P<0.01);Ludmila Belayev 12分评分法评分与模型组比较,莫诺苷大剂量组差异极显著(P<0.001),中剂量组差异极显著(P<0.01)。结论莫诺苷对局灶性脑缺血再灌注模型大鼠有改善行为学评分的作用。

Objective To investigate the effect of Morroniside which was isolated from Cornus of ficinalis on recovery of neurological function after focal cerebral ischemia reperfusion in rats.Methods Wistar male rats were randomly divided into sham operated group,model group and Morroniside-low（30 mg/kg）,Morroniside-middle（90 mg/kg）,Morroniside-high（270 mg/kg）,vitamin E（35 mg/kg）.Focal stroke was induced in adult rats by 30 minutes right middle cerebral artery occlusion reperfusion injury（tMCAO/R）.To examine the recovery of neurological function,we measured Zea Longa＇s method,Grid test,Beam-walking test,Prehensile traction test,Ludmila Belayev＇s method on the third day after operations in rats.Result The above mentioned indexes,including Zea Longa＇s method,Prehensile traction test in the Morroniside-treated group showed differences compared with those in the model group（P0.01） on the third day after the right middle cerebral artery occlusion reperfusion injury （tMCAO/R） in rat,and Ludmila Belayev＇s method in the Morroniside-treated group showed differences compared with those in the model group（P0.001）.Conclusion（Morroniside ameliorate） focal cerebral ischaemia-reperfusion injury induced neurological function deficits in Rats.