期刊文献+

茉莉花水提物对大鼠胸主动脉的舒张作用及机制 被引量:8

Vasodilation Effect of Aqueous Extract of Jasmine on Rat Thoracic Aorta and Its Related Mechanism
原文传递
导出
摘要 目的观察中药茉莉花水提物(AEJ)的血管舒张效应并探讨其机制。方法采用大鼠胸主动咏环灌流,记录张力的变化;激光扫描共聚焦显微镜技术检测血管平滑肌细胞内Ca^2+浓度。结果AEJ(0.375~6g·L^-1)能够浓度依赖性降低苯肾上腺素(PE,10μmol·L^-1)及KCl(60mmol·L^-1)引起的主动脉环张力。在无钙环境下,AEJ能抑制高浓度氯化钾(KCI60mmol·L^-1)环境下累计加入CaCl2(0.5~8mmol·L^-1)引起的收缩,抑制PE(10μmol·L^-1)引起的去内皮主动脉环的短暂收缩(P〈0.01)。钾通道阻断剂4-氨基吡啶(5mmol·L^-1)可显著抑制AEJ的舒血管作用。激光扫描共聚焦检测细胞内Ca^2+的结果表明,AEJ浓度依赖性(6,12g·L^-1)降低了KCI除极诱导的滑肌细胞胞浆内Ca^2+的升高幅度(P〈0.05)。结论AEJ能够浓度依赖性舒张大鼠胸主动脉,其作用机制可能是减少Ca^2+经电压依赖性钙通道和受体操纵性钙通道流入血管平滑肌细胞及抑制内质网内Ca^2+释放有关;电压敏感型K+通道(Kv)的激活部分参与了AEJ舒血管作用。 OBJECTIVE To investigate the vasodilative effect of aqueous extract of Jasmine ( A EJ ) , a kind of Chinese materia medica, and the possible mechanism. METHODS Isometric tension was recorded in isolated rat thoracic artery- to study the vasodilation effect of AEJ. [ Ca^2+ ] was determined by laser scanning confocal microscope (LSCM). RESULTS AEJ (0. 375 - 6 g · L^-1 ) caused concentration-dependent relaxation of endothelium-intact or endothelium-denuded aorta tings precontracted with phenylephrine (PE, 10 μmol· L ^-1) or KCl (60 mmol· L^-1 ). In Ca^2+ -free medium, AEJ decreased Ca^2+ -dependent (0. 5 -8 mmol· L^-1 ) tension of thoracic aorta induced by high concentration of K + ( 60 mmol· L^-1 ). It also reduced the contraction elicited by PE in Ca^2+ -free medium (P 〈0. 01 ). 4-Aminopyridine(4-AP)(5 mmol· L^-1), a voltage-dependent K+ channel inhibitor, attenuated the vasorelaxingeffectofAEJ(P〈0.01). LSCM showed that [Ca2+]increased by KCl was depressed by AEJ(6, 12 g· L^-1)(P〈0.05). CONCLUSION AEJ exerted a dose-dependent vasorelaxant effect on rat isolated aorta rings. The mechanism is related to the inhibition of voltage-dependent Ca^2+ channel (VDC) , rec, eptor-operate Ca^2+ channel (ROC) and release of Ca^2 + from sarcoplasmic reticulure. The activation of Kv contribute partly to the endothelium-independent relaxation by AEJ.
出处 《中国药学杂志》 CAS CSCD 北大核心 2010年第3期182-186,共5页 Chinese Pharmaceutical Journal
基金 国家科技支撑计划资助课题(2006BAD27B05) 哈尔滨市科委科技创新人才研究专项基金(2009RFXXS005)
关键词 茉莉花 血管环 血管舒张 钙通道 电压敏感型K+通道 激光扫描共聚焦 jasmine aorta rings vasodilatation Ca^2+ channel voltage-dependent K^+ channel laser scanning confocal microscope
  • 引文网络
  • 相关文献

参考文献10

  • 1ASHRAF M Z, HUSSAIN M E, FAEHIM M. Endothelium mediated vasorelaxant response of garlicin isolated rat aorta: role of nitric oxide [ J]. Ethnopharmacolog, 2004, 90 (1):5-9.
  • 2王旭慧,朱亮,陈红.黄芪甲苷对正常大鼠离体血管功能的影响[J].中国药理学通报,2006,22(11):1319-1324. 被引量:34
  • 3田霞,王逸平.丹酚酸B镁抑制ATP和KCl诱导大鼠主动脉平滑肌细胞内钙的升高[J].中国药理学通报,2006,22(3):311-316. 被引量:18
  • 4李家富,章茂顺,王家良,符宗胤,廖晓阳.槲皮素对家兔主动脉血管平滑肌细胞胞内游离钙浓度的影响[J].高血压杂志,2000,8(1):55-57. 被引量:24
  • 5RAPOPORT R M, MURAD F. Agonist - induced endothelium - dependent relaxation in rat thoracic aorta may be mediated through cGMP[J]. Circ Res, 1983 52 (3) :352-357.
  • 6VAANDRAGER A B , DE JONGE H R. Signalling by cGM dependent protein kinases [ J]. Mol Cell Biochem, 1996,157 ( 1- 2) :23-30.
  • 7BEAN B P. Classes of calcium channels in vertebrate cells[ J].Annu Rev Physiol, 1989, 51 : 367-384.
  • 8REMBOLD C M. Regulation of contraction and relaxation in arterial smooth mascle [ J ]. Hypertension, 1992, 20 ( 2 ) : 129-137.
  • 9JACKSON W F. Ion channels and vascular ton [ J]. Hypertension, 2000, 35 (1 Pt 2):173-178.
  • 10NELSON M T, QUAYLE J M. Physiological roles and properties of potassium channels in arterial smooth muscle[ J]. Am J Physiol, 1995, 268 (4 Pt1 ) :799-822.

二级参考文献34

共引文献65

同被引文献104

引证文献8

二级引证文献31

;
使用帮助 返回顶部