摘要
目的观察中药茉莉花水提物(AEJ)的血管舒张效应并探讨其机制。方法采用大鼠胸主动咏环灌流,记录张力的变化;激光扫描共聚焦显微镜技术检测血管平滑肌细胞内Ca^2+浓度。结果AEJ(0.375~6g·L^-1)能够浓度依赖性降低苯肾上腺素(PE,10μmol·L^-1)及KCl(60mmol·L^-1)引起的主动脉环张力。在无钙环境下,AEJ能抑制高浓度氯化钾(KCI60mmol·L^-1)环境下累计加入CaCl2(0.5~8mmol·L^-1)引起的收缩,抑制PE(10μmol·L^-1)引起的去内皮主动脉环的短暂收缩(P〈0.01)。钾通道阻断剂4-氨基吡啶(5mmol·L^-1)可显著抑制AEJ的舒血管作用。激光扫描共聚焦检测细胞内Ca^2+的结果表明,AEJ浓度依赖性(6,12g·L^-1)降低了KCI除极诱导的滑肌细胞胞浆内Ca^2+的升高幅度(P〈0.05)。结论AEJ能够浓度依赖性舒张大鼠胸主动脉,其作用机制可能是减少Ca^2+经电压依赖性钙通道和受体操纵性钙通道流入血管平滑肌细胞及抑制内质网内Ca^2+释放有关;电压敏感型K+通道(Kv)的激活部分参与了AEJ舒血管作用。
OBJECTIVE To investigate the vasodilative effect of aqueous extract of Jasmine ( A EJ ) , a kind of Chinese materia medica, and the possible mechanism. METHODS Isometric tension was recorded in isolated rat thoracic artery- to study the vasodilation effect of AEJ. [ Ca^2+ ] was determined by laser scanning confocal microscope (LSCM). RESULTS AEJ (0. 375 - 6 g · L^-1 ) caused concentration-dependent relaxation of endothelium-intact or endothelium-denuded aorta tings precontracted with phenylephrine (PE, 10 μmol· L ^-1) or KCl (60 mmol· L^-1 ). In Ca^2+ -free medium, AEJ decreased Ca^2+ -dependent (0. 5 -8 mmol· L^-1 ) tension of thoracic aorta induced by high concentration of K + ( 60 mmol· L^-1 ). It also reduced the contraction elicited by PE in Ca^2+ -free medium (P 〈0. 01 ). 4-Aminopyridine(4-AP)(5 mmol· L^-1), a voltage-dependent K+ channel inhibitor, attenuated the vasorelaxingeffectofAEJ(P〈0.01). LSCM showed that [Ca2+]increased by KCl was depressed by AEJ(6, 12 g· L^-1)(P〈0.05). CONCLUSION AEJ exerted a dose-dependent vasorelaxant effect on rat isolated aorta rings. The mechanism is related to the inhibition of voltage-dependent Ca^2+ channel (VDC) , rec, eptor-operate Ca^2+ channel (ROC) and release of Ca^2 + from sarcoplasmic reticulure. The activation of Kv contribute partly to the endothelium-independent relaxation by AEJ.
出处
《中国药学杂志》
CAS
CSCD
北大核心
2010年第3期182-186,共5页
Chinese Pharmaceutical Journal
基金
国家科技支撑计划资助课题(2006BAD27B05)
哈尔滨市科委科技创新人才研究专项基金(2009RFXXS005)
关键词
茉莉花
血管环
血管舒张
钙通道
电压敏感型K+通道
激光扫描共聚焦
jasmine
aorta rings
vasodilatation
Ca^2+ channel
voltage-dependent K^+ channel
laser scanning confocal microscope