HPLC法测定云南白药中三七皂苷R_1、人参皂苷Rg_1及人参皂苷Rb_1含量

Determination of Notoginsenoside R_1 and Ginsenoside Rg_1、Rb_1 in Yunnan Baiyao by HPLC

目的:建立高效液相色谱法同时测定云南白药中三七皂苷R_1、人参皂苷Rg_1、Rb_1含量方法。方法:采用ShimadzuVP-ODS C_(18)色谱柱(150 mm×4.6 mm,5μm),流动相:乙腈-水梯度洗脱;流速为1.0 ml·min^(-1);检测波长为203 nm。结果:三七皂苷R_1、人参皂苷Rg_1及Rb_1的线性范围分别为0.12～0.81μg,r=0.999 9,1.31～9.17μg,r=0.999 9及0.13～0.91μg,r=0.999 8,回收率分别为:98.9%(RSD=2.55%),98.7%(RSD=1.26%)及99.3%(RSD=1.60%)。结论:方法简便、准确、重复性好,可用于该制剂的质量控制。

Objective ： To establish an HPLC method for the determination notoginsenoside R1 and ginsenoside Rg1 , RbI in Yunnan Baiyao. Method： The Shimadzu VP-ODS C 18 Column （150 mm× 4. 6 mm,5μm） was used, the mobile phase consisted of acetonitrile- water with gradient elution, the flow rate was 1.0 ml. min - 1, the detection wavelength was 203 nm. Result： The linear ranges of notogin- senoside R1 and ginsenoside Rg1 ,Rb1 were 0.12-0.81 μg,r =0. 999 9,1.31-9.17 μg,r =0.999 9 and 0.13-0.91 μg,r =0.999 8 ,the average recovevies （n =6） were 98.9% （RSD =2. 55% ） ,98. 7% （RSD = 1.26% ） ,99. 3% （RSD = 1.60% ） ,respectively. Conclusion： The method is simple, accurate and repeatable,it can be used for the quality control of yunna baiyao.