邻菲咯啉钌(Ⅱ)化学发光分析测定环丙沙星

TRIS (1,1 PHENANTHROLINE)RUTHENIUM(Ⅱ) CHEMILUMINESCENCE DETERMINATION OF CIPROFLOXACIN IN PHARMACEUTICAL PREPARATION

试验表明，在０．０１ｍｏｌＬ－１ＮａＡｃ－ＨＡｃ（ｐＨ５．０）底液中，环丙沙星能增强Ｃｅ（Ⅳ）氧化Ｒｕ（ｐｈｅｎ）２＋３的化学发光强度，据此建立了环丙沙星的化学发光分析法，线性范围为３．６×１０－７～１．２×１０－５ｇｍＬ－１，检测限为４．４×１０－８ｇｍＬ－１，对８×１０－７ｇｍＬ－１和５×１０－５ｇｍＬ－１的环丙沙星平行测定９次，其相对标准偏差分别为２．８％和３．３％，该法用于片剂中环丙沙星含量的测定，获得满意结果．

According to the mechnism of the reaction of tris (1,10 phenanthroline) Ru(Ⅱ) (Ru(phen) 2+ 3) organic acids Ce(Ⅳ), ciprofloxacin (CF) should be able to determine by this method. It is proved that the CL intensity is enhanced by CF in 0.01 mol·L -1 NaAc HAc(pH 5.0). The linear range and detection limit are 3.6×10 -7 1.2×10 -5 g·mL -1 and 4.4×10 -8 g·mL -1 , respectively. The method has been satisfactorily applied to determine CF in table samples.