hPF4 mRNA表达、血浆PF4水平与甲状腺乳头状癌颈部淋巴结转移的关系

Relationship between Expression of hPF4 mRNA,Concentration of Plasma PF4 and Cervical Lymph Node Metastasis in Papillary Thyroid Carcinoma

目的探讨hPF4 mRNA表达、血浆PF4水平与甲状腺乳头状癌(PTC)颈部淋巴结转移的关系。方法应用逆转录多聚酶链反应(RT-PCR)技术检测40例PTC癌组织和10例癌旁正常组织hPF4 mRNA的表达,并用酶标记免疫分析法(ELISA)测定40例PTC患者、20例健康体检者(对照组)的血浆PF4水平。结果(1)PTC癌组织hPF4 mRNA阳性表达率分别为47.5%,明显低于正常组织(80%)(P<0.01),而且hPF4 mRNA阳性表达PTC组的颈部淋巴结转移率均明显低于hPF4阴性表达PTC组(36.84%vs.90.48%,P<0.01)。(2)正常对照组、无淋巴结转移的PTC组和伴淋巴结转移的PTC组间的PF4血浆浓度呈线性递减趋势,其中无淋巴结转移组的PF4血浆浓度[(2.96±0.21)ng/ml]高于转移组[(0.98±0.17)ng/ml](P<0.05)。(3)hPF4 mRNA阳性表达PTC组的血浆PF4水平明显高于hPF4阴性表达组[(2.49±0.83)ng/mlvs.0.94±0.13)ng/ml](P<0.01)。结论PTC患者血浆及局部癌灶PF4水平的下调可能是导致或助推PTC发生颈部淋巴结癌转移的成因之一。

Objective To explore the relationship between expression of human platelet factor 4（hPF4）mRNA, concentration of Plasma PF4 and cervical lymph node metastasis in papillary thyroid carcinoma（PTC）. Methods RT-PCR technique was utilized to detect the expression of hPF4 mRNA in 40 cases of PTC and 10 cases of normal tissue surrounding carcinoma. Meanwhile enzyme-linked immunosorbant assay（ELISA） technique was used to measure the concentration of PF4 in plasma of all 40 patients with PTC and 20 healthy persons as control. Results （1）The positive expression rate of hPF4 mRNA of PTC and normal tissues was 47.5% and 80%,respectively. The positive expression rate of hPF4 mRNA of PTC tissue was significantly lower than that of normal tissues（P〈0.01）. Furthermore the metastatic rate of cervical lymph node of PTC with hPF4 mRNA positive expression was significantly lower than that of PTC with hPF4 mRNA negative expression （36.84% vs. 90.48%）（P〈0.01）. （2） The concentration of PF4 in plasma of normal healthy person, PTC with cervical lymph node metastasis and PTC without cervical lymph node metastasis was in linear progressive decreasing tendency. The plasma concentration of PF4 in PTC without cervical lymph node metastasis[（2.96±0.21）ng/ml] was higher than that in PTC with cervical lymph node metastasis[（0.98±0.17）ng/ml]（P〈0.05）. （3） The plasma concentration of PF4 in PTC with hPF4 mRNA positive expression was higher than that in PTC with hPF4 mRNA negative expression[（2.49±0.83）ng/ml vs. （0.94±0.13）ng/ml]（P〈0.01）. Conclusion The decline of the level of plasma PF4 and local lesion of PTC may be one of the reasons which can cause or promote cervical lymph node metastasis of PTC.