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L-谷氨酰肼为底物酶法制备茶氨酸 被引量:5

Enzymatic Synthesis of Theanine with L-Glutamlhydrine as Substrate
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摘要 采用一种廉价的底物L-谷氨酰肼代替L-谷氨酰胺,成功地合成了茶氨酸,并且对该酶促反应的条件进行了初步优化。结果表明,以L-谷氨酰肼为底物时,γ-谷氨酰转肽酶(GGT)活性约是以L-谷氨酰胺为底物时的78.3%。该酶促反应的最适条件为:pH=10,反应温度40℃,n(L-谷氨酰肼)∶n(乙胺)=1∶10,L-谷氨酰肼初始浓度0.3mol/L。利用以上条件进行了茶氨酸的小量制备,投料L-谷氨酰肼50g,乙胺140g,反应40h,L-谷氨酰肼的摩尔转化率达84.1%,经离子交换树脂分离产物,得到31.8g高纯度茶氨酸。该结果显示良好应用前景。 In this research, an economical substrate, L-glutamlhydrine, was used in theanine synthesis catalyzed by Escherichia coli cells with L-glutamyhranspeptidase(GGT) activity. The result showed that GGT activity with L-glutamlhydrine as substrate was about 78. 3% of that with L-glutamine as substrate. In the optimized reaction condition with 0. 3 mol/L L-glutamlhydrine,3 mol/L ethylamine and 0. 018 g/ mL of GGT cells at pH = 10 and 40 ℃,0. 25 mol/L theanine was obtained in 40 h. The conversion rate from L-glutamlhydrine to theanine was 84. 1%. Theanine of high purity was obtained by ion exchange resin. The results exhibited a good prospect of application.
出处 《精细化工》 CAS CSCD 北大核心 2010年第2期130-132,141,共4页 Fine Chemicals
基金 国家技术创新基金资助项目(02CJ-13-01-16)~~
关键词 L-谷氨酰肼 茶氨酸 Γ-谷氨酰转肽酶 酶法合成 生物工程 L-glutamlhydrine theanine γ-glutamyhranspeptidase enzymatic synthesis biological engineering
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参考文献19

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