276例疑似生殖器疱疹患者单纯疱疹病毒Ⅱ型检测结果分析

Analysis of 276 cases of genital herpes in patients with suspected herpes simplex virus type Ⅱ

目的用实时荧光定量聚合酶链反应(FQ-PCR)检测我院皮肤性病门诊生殖器疱疹疑似患者的单纯疱疹病毒Ⅱ型(HSV-Ⅱ)感染情况。方法用FQ-PCR对276例生殖器疱疹疑似患者进行检测。结果①276例生殖器疱疹疑似患者检测HSV-Ⅱ阳性患者145例,阳性率为52.53%(145/276),其中男性患者阳性率59.76%(101/169),女性患者41.12%(44/107),男性患者与女性患者的发病率比较,差异具有统计学意义(P<0.05)。②三个年龄段患者即<20岁,20～55岁,>55岁的阳性率分别为31.25%(5/16),62.62%(129/206),20.37%(11/54),三个年龄段患者发病率比较,差异均具有统计学意义(P<0.05)。结论①生殖器疱疹疑似患者中HSV-Ⅱ感染率高,感染率有性别差异,男性发病率高于女性。②在不同的年龄发病率不同。③FQ-PCR在单纯疱疹病毒检测中具有快速,简便,灵敏度高等优点,适用于临床检测。

Objective To investigate the prevalece of herpes simplex virus type Ⅱ（HSV Ⅱ ） in the patients attending a sexually transmitted diseases clinic, and to investigate the positive rate of HSV 1/ in these patients with typical and atypical genital herpes （GH）. Methods Fluorescence quantitative polymerase reaction technology （FQ-PCR） was used to detect HSV Ⅱin 276 patients with typical and atypical genital herpes （GH）. Results （1）Among 276 patients, HSV 11 infection was founded in 145 patients （52.53%）. The positive detection rate was 59.76% in men and 41.12% in women patients.There was significant difference between the positive detection rate of men and women （P〈0.05）. （2）Three age groups were age 〈20,20-55, 〉55, the positive detection rate of this three age groups was 31.25% （5/16） , 62.62% （129/206） , 20.37% （11/54） respectively. There was significant difference in this three groups （P〈0.05）. Conclusion （1）There is higher infection rate of HSV in the area, the HSV Ⅱ infection was dominated. There was significant difference of positive detection rate between men and women. Men is higher than women. （2） There was different positive detection rate of different age groups. （3）FQ-PCR is a rapid, easy, sensitive and special assay to detect HSV in patients with atypical GH, it can be applied for clinical diagnosis.