HPLC法测定人参芦中人参皂苷Rb_1、Rg_1、Re的含量

Determination of Ginsenoside Rb_1,Rg_1 and Re in Ren Shen Lu by HPLC

目的:建立人参芦中人参皂苷Rb_1、Rg_1和Re的含量测定HPLC方法。方法:Agilent Zorbax ODS C_(18)色谱柱(250 mm×4.6 mm,5μm),柱前加保护柱,检测波长为203 nm;流动相为乙腈-水,梯度洗脱;流速:1.0ml·min^(-1);柱温:27℃;结果:结果表明人参皂苷Rb_1在1.0～10.1μg范围内呈良好的线性关系,r=0.999 8;平均回收率为99.7%,RSD=1.96%(n=5);人参皂苷Rg_1在1.0～10.1μg范围内呈良好的线性关系,r=0.999 7,平均回收率为99.9%,RSD=1.54%(n=5);人参皂苷Re在0.69～6.90μg范围内呈良好的线性关系,r=0.999 4,平均回收率为99.5%,RSD=1.58%(n=5)。结论:该法简便、准确,可作为人参芦的质量控制。

Objective： To establish an HPLC method for determination ginsenoside RbI , Rg1 and Re in Ren shen lu. Method： The Angilent Zorbax ODS Cls columm （250 mm x4. 6 mm,5 tun） was used,the detection wavelength was 203 nm,the mobile phase consis- ted of acetonitrile-water,gradient elution the＇flow rate was 1.0 ml.min-1, the column temperature was 27 ℃. Result： The linear ranges of ginsenoside RbI , Rg1 and Re were 1.0-10. 1μg,r =0. 999 8,1.0-10. 1 μg,r =0. 999 7 and 0. 69-6. 90 μg,r =0. 999 4;the average recoveries （n =5） were 99.7% （RSD 1.96% ）. 99.9% （RSD 1.54% ） and 99. 5% （RSD 1.58% ） respectively. Conclusion： The method is simple and accurate,it can be used for quality control of Ren Shen Lu.