L-赖氨酸产生菌FH128菌株的研究(Ⅰ)FH128菌株的选育及摇瓶发酵

Studies on L Lysine Producing Strain FH128 (Ⅰ) The Screening of L Lysine Producing Strain FH128 and Shake Flask Culture

以赖氨酸产生菌Ａ１１１（ＨＳ－、ＡＥＣｒ）为出发株，经化学诱变剂ＭＮＮＧ（Ｎ－甲基－Ｎ′－硝基－Ｎ－亚硝基胍）及单氟醋酸处理获得单氟醋酸抗性突变株Ｆ７９，摇瓶发酵产Ｌ－赖氨酸盐酸盐７．０％～７．５％，对糖转化率３８％～４０％，分别比Ａ１１１株提高约２５％及２０％。然后，再以Ｆ７９菌为亲株经ＭＮＮＧ及噻唑丙氨酸处理获得噻唑丙氨酸抗性突变株ＦＨ１２８，在适宜的培养条件下，摇瓶发酵产Ｌ－赖氨酸盐酸盐８．５％～９．５％，最高产酸率１１％，对糖转化率４５％～５０％。在１６Ｌ自控发酵罐发酵，产Ｌ－赖氨酸盐酸盐１２％～１４％，对糖转化率４０％～４５％；在２０～１００ｍ３发酵罐发酵，产酸率为８．５％～９．５％，对糖转化率４０％～４２％，提取总收率８０％～８５％，成品（饲料级Ｌ－赖氨酸盐酸盐）质量符合国家标准（ＧＢ８２４５－８７）。ＦＨ１２８菌株遗传性能稳定，营养要求粗放，工艺较简单，便于工业化，二年前已应用于工业生产。

A resistant mutant of F79,the lysine producer, was derived from the parent strain A111(HS -、AEC r) by mutation with MNNG(N methyl N′ nitro N nitrosoguanidine) and MF(Monfluoroactic acid).The production of L lysine·HCl of the mutant in flask was 7.0%￣7.5%,the conversion rate of glucose to L lysine was 38%￣40%.The TA(α Thiazol DL Alanine) resistant strain FH128 was derived from the parent strain F79 by mutation with MNNG and TA.Under optimal culture coditions,the production of L lysine·HCl was 8.5%￣ 9.5%; the maxium production of L lysine·HCl was 11%,the conversion rate of glucose was 45%￣50% in shake flask.In 16 L fermenter,the production of L lysione·HCl was 12%￣14%,the coversion rate was 40%￣45%.In 20 m 3￣100 m 3 fermenter,the production of L lysine·HCl was 8.5%￣9.5%,the conversion rate was 40%￣42%.