高粱全基因组生长素原初响应基因Aux/IAA的序列特征分析

Whole-Genome Sequence Characterization of Primary Auxin-Responsive Aux/IAA Gene Family in Sorghum (Sorghum bicolor L.)

生长素在生物体的生长、发育与分化过程中发挥着重要作用。Aux/IAA基因家族在生长素诱导的早期做出响应,是生长素早期应答的三大基因家族之一。采用生物信息学的方法,对高粱全基因组Aux/IAA基因进行序列特征分析表明,高粱基因组中共有25个Aux/IAA基因,分布于高粱9对染色体,在高粱第6号染色体上未发现Aux/IAA基因。序列比对与系统发生分析表明,22个(88%)高粱Aux/IAA蛋白具有4个保守结构域,19对高粱/玉米Aux/IAA蛋白、2对高粱/水稻Aux/IAA蛋白处于系统进化树的同一分支。该研究结果不仅有助于生长素信号转导通路的解析,而且可以为其他作物基因家族的研究提供参考。

The phytohormone auxin plays an important role in organism growth,development and differentiation. Members of Aux/IAA gene family are responsive primarily to auxin induction. Despite the physiological importance,sequence charac-terization of Aux/IAA genes has not yet been reported in sorghum. Herein,we presented the isolation and characterization of Aux/IAA genes from sorghum using bioinformatic method. A total of 25 Aux/IAA gene family members were distributed on ninth chromosomes in sorghum except Chr. 6. Sequence alignment and phylogenetic analysis showed major sorghum Aux/IAA proteins shared four conserved domains. There were nineteen pairs of sorghum / maize and two pairs of sorghum / rice Aux/IAA proteins which were in the same clades of the dendrogram. Aux/IAA proteins partly demonstrated in maize is the closest relative of sorghum in the grass family. The results will be helpful to the auxin signal transduction cascade investigation and gene family research in other crops.