摘要
目的:研究黄连解毒汤及其单味药对细胞氧化损伤保护作用的药效动力学,并对其血清药理学方法进行探讨。方法:采用血清药理学方法,研究黄连、黄芩、黄连解毒汤含药血清对H2O2所致PC12细胞氧化应激损伤保护作用的时间-效应关系;并研究黄连、黄芩、黄连解毒汤提取物和小檗碱、黄芩苷纯品对氧化损伤保护作用的剂量-效应关系。结果:各药的含药血清对细胞氧化损伤都有明显的保护作用,其中以黄连解毒汤作用最强,黄芩和黄芩苷次之,黄连最弱;在试验浓度范围内,黄连解毒汤和黄芩提取物对模型细胞有剂量依赖性保护作用,而黄连没有保护作用;在0.15~10μg.mL-1浓度范围内,黄芩苷对细胞模型有保护作用,且呈剂量依赖性。0.15~10 ng.mL-1浓度范围内,小檗碱没有明显的保护作用。结论:黄连解毒汤体内抗氧化作用强于各单味药;黄芩苷可能是黄芩及其含药血清中的抗氧化成份之一,而小檗碱与含药血清的抗氧化作用无关;黄连解毒汤及其单味药含药血清对细胞氧化应激模型的保护作用呈时间和剂量依赖性。
Objective:To study protective effects of Huanglian Jiedu Tang(HLJDT) and its components Coptidis Rhiyoma(CR),Scutellariae Redix(SR) on oxidative stress-induced PC12 cell injury.Methods: Serum pharmacological method was used to study effects of HLJDT,CR,SR on the cell oxidative injury.The herb-contained serum,obtained at different time after dosing in rats,was added to the cultured oxidative PC12 cells.The viability of PC12 cells was determined by MTT colormetric microassay.Effects of decoctions of HLJDT,CR,SR,herberine and baicalin were investigated as well.Result: The herb-contained serum of HLJDT,SR,CR,baicalin and the decoctions of HLJDT,SR,baicalin had protective effects on cell injury so did baicalin at 0.15 ~ 10 μg.mL-1.But herberine and the decoctions of CR had no effect.Conclusion: Obtained serum after ig.HLJDT and its components in rats have protective effect on oxidative stress-induced PC12 cell injury in a dose-dependent and time-dependent manner.Baicalin may act as an antioxidant in the herb-contained serum of HLJDT as well as in the decoctions.
出处
《中国实验方剂学杂志》
CAS
北大核心
2010年第4期118-122,共5页
Chinese Journal of Experimental Traditional Medical Formulae
关键词
黄连解毒汤
血清药理学
PC12细胞
过氧化氢
氧化应激损伤
Huanglian Jiedu Tang
serum pharmacology
PC12 cell
hydrogen peroxide
oxidative stress-induced injury