艾叶的体外凝血作用实验研究

Experiment study on external hemagglutination test of argy wormwood leaf

[目的]传统中医常用艾炭作为止血药,且具有较好的止血作用,探讨止血的原理。[方法]将艾叶不同组分进行分离,或按传统方法炮制,得到6种组分:鞣酸、艾焦油、5-叔丁基连苯三酚、艾炭、艾灰、艾叶挥发油。用家兔分别进行体外凝血实验,得出实验结果。[结果]凝血作用强弱顺序为鞣酸>艾焦油>5-叔丁基连苯三酚>艾炭>艾灰>艾叶挥发油。其中添加挥发油凝血时间长于对照品生理盐水,故为活血组分。[结论]中医采用艾炭止血有一定的科学性,但是艾炭的凝血效果是最差的,艾叶鞣酸才是真正的凝血物质。为了解艾炭的止血原理,开发新的止血药创造了条件。

[Objective] Argy wormwood leaf carbon was commonly used as a hemostatic by traditional Chinese doctors because it showed a better role in hemostasis. We conducted an experiment in order to investigate the principles of hemostasis. [Methods] After separating the different components of Artemsia argyi or concocting them by traditional methods, we separated 6 kind of components： tannin, Artemsia argyi tar, 5-tert-butyl trihydroxybenzene, Artemsia argyi carbon, Artemsia argyi ash and Artemsia argyi volatile oil. Their coagulation effect then detected in vitro. [Results] The results showed that the most effective component was tannin, followed by Artemsia argyi tar, 5- tert-butyl trihydroxybenzene, Artemsia argyi carbon, Artemsia argyi ash, and Artemsia argyi volatile oil. Among them. Artemsia argyi volatile oil had some blood-activating component since it could prolong the clotting time if adding. [Conclusion] Our experiment has established the conditions for understanding the processing mechanism of Artemsia argyi carbon and developed a new hemostatic. The most effective component was tannin.