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米非司酮对人早孕绒毛细胞增殖和凋亡的影响 被引量:183

Effects of Mifepristone on Proliferation and Apoptosis in Early Chorionic Villi
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摘要 目的探讨米非司酮对人早孕绒毛滋养细胞增殖和凋亡的影响。方法对15例正常早孕吸宫流产和15例米非司酮流产病例的绒毛组织,应用免疫组织化学ABC法检测增殖细胞核抗原(PCNA)在细胞中的染色(细胞增殖)情况,DNA缺口原位末端标记法检测细胞凋亡。结果正常早孕绒毛细胞滋养细胞增殖指数为53.74%±1.34%,且见细胞浆阳性染色,合体滋养细胞未见PCNA阳性染色;合体滋养细胞和细胞滋养细胞的凋亡指数分别为2.52%±0.86%和0.52%±0.26%;米非司酮流产绒毛细胞滋养细胞增殖指数降为50.86%±1.44%,细胞浆阳性现象消失;细胞凋亡指数显著增加,合体滋养细胞和细胞滋养细胞分别达到22.16%±2.26%和20.12%±1.74%。 Objective To study the effects of mifepristone on proliferation and apoptosis in early pregnant chorionic villi. Methods Proliferation and apoptosis in early pregnant choionic villi from surgical aspiration and mifepristone induced abortion were studied. Marker for proliferation is proliferating cell nuclear antigen (PCNA)immunohistochemical staining, apoptotic cell death was detected using DNA in situ terminal deoxynucleotidyl transferas mediated dUTP biotin nick ending labeling(TUNEL). Results In early pregnant chorionic villi, proliferating index of cytotrophoblasts was 53.74%±1.34%, not only nuclear but also cytoplasma were PCNA positive staining,syncytiotrophoblasts were negative. There existed apoptotic cell death in trophoblasts,the apoptotic index were 2.52%± 0.86% in syncytiotrophoblasts and 0.52%±0.26% in cytotrophblasts, respectively. After 2 days mifepristone treatment, the proliferating index of cytotrophoblasts decreased slightly and PCNA positive cytoplasmic expression disappeared; while apoptotic cell increased significantly, the apoptotic indices were 22.16%±2.26% in syncytiotrophoblasts and 20.12%±1.74% in cytotrophoblasts, respectively. Conclusion Mifepristone may inhibit proliferation and promote apoptosis of trophoblasts in early pregnant chorionic villi.
出处 《中华妇产科杂志》 CAS CSCD 北大核心 1998年第5期268-270,I008,共4页 Chinese Journal of Obstetrics and Gynecology
关键词 米非司酮 绒毛膜绒毛 脱噬作用 终止妊娠 Mifepristone Chorionic villi Apoptosis
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参考文献3

  • 1刘伯宁,中华妇产科杂志,1995年,30卷,7页
  • 2Shi W L,Fertil Steril,1993年,60卷,68页
  • 3唐敏一,胎盘病理学,1987年,5页

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