浙江汉族人群HLA—DPA1和-DPB1基因多态性分析

Analysis of the HLA-DPA1 and HLA-DPB1 polymorpbism of Zhejiang Han population by PCR-sequence based typing

目的检测浙江地区汉族人群HLA-DPA1和HLA—DPB1等位基因及单倍型频率。方法应用PCR-直接测序分型法对100名健康、无血缘关系的浙江汉族人外周血标本进行HLA—DPA1和HLADPB1等位基因分析。结果在100份标本中检出8个HLA-DPA1等位基因和19个HLA—DPB1等位基因。HLA—DPA1等位基因频率较高的依次为DPA1＊020202（47．0％）、DPA1＊010301（38．5％）和DPA1＊020101（10．5％）。HLA-DPB1等位基因中，频率较高的依次为DPB1＊0501（39．5％）、DPB1＊020102（13．5％）和DPB1＊040101（13．0％）。连锁分析发现共有44个HLA-DPA1-DPB1单倍型，单倍型频率最高为DPA1＊020202-DPB1＊0501（29．5％）。结论浙江地区汉族人群HLA～DPA1和～DPB1基因座等位基因具有丰富的多态性，2个基因座呈现连锁不平衡。

Objective To investigate the polymorphisms of HLA-DPA1 and HLA DPB1 loci of Han population in Zhejiang province of China. Methods The alleles of HLA-DPA1 and HLA-DPB1 loci in 100 unrelated healthy individuals were analyzed using polymerase chain reaction sequence based typing. Results Eight HLA-DPA1 alleles and 19 HLA-DPB1 alleles were found in the population. The HLA-DPA1 alleles with higher frequencies were DPA1 ＊020202 （47. 0%）, DPA1 ＊ 010301 （38. 5%） and DPA1 020101（10. 5%）. The HLA-DPB1 alleles with higher frequencies were DPBJ ＊ 0501, DPB1 ＊ 020102 and DPB1 ＊ 040101. The frequencies were 39. 5%, 13. 5% and 13.0%, respectively. A total of 44 estimated DPA1-DPB1 haplotypes were detected. The HLA-DPA1 ＊ 020202 DPB1 ＊ 0501 （29. 50/oo） was the most frequent haplotype. Conclusion The polymorphism data of the HLA-DPA1 and -DPB1 were obtained in Han population in Zhejiang province of China. There was linkage disequilibrium between the two loci.