辐射八宝景天ISSR遗传多样性分析

Analysis on the Genetic Diversity of Radiated Sedum spectabile Bareau by ISSR

为研究辐射所得八宝景天植株的遗传多样性，采用ISSR分子标记技术对0、30、50Gy3个梯度辐射所得的6株八宝景天突变体的亲缘关系进行分析，以确定突变体之间的遗传差异。结果表明：23条ISSR引物能在6个植株间扩增出175条谱带，其中127条是多态性谱带，多态率高达72．57％，表现出了较高的多态性。从聚类树状图中可以看出，6个植株间的相似系数在0．5805-0．7886，平均相似系数为0．6846，对照植株和辐射植株有一定的差异性。随着辐射剂量的增大，遗传距离增大，相似系数减小，相应的变异系数增大。

The aim was to discuss the genetic diversity of Sedum spectabile Bareau obtained by radiation. The genetic relationship of Sedum spectabile Bareau obtained by radiation with different gradient（0,30,50 GY） was analyzed by ISSR molecularmarker technology to determine the genetic diversity among these mutants. 175 bands were amplified with 23 ISSR primers among 6 kinds of Sedum spectabile Bareau, of which, 127 bands were polymorphic and the polymorphism rate reached to 72. 57%, which showed higher polyrnorphism. The dendrogram showed that the similarity coefficient among 6 kinds of plants were 0. 5805 - 0. 7886 and the average similarity coefficient was 0. 6846. There were certain differences on the similarity coefficient between the control plant and radiated plants. The genetic distance between the radiated sample and the control sample was increased, the similarity coefficient was decreased and the corresponding variation coefficient was increased with the increment of the radiation dose.