电针12h对颅脑损伤大鼠脑组织[Ca^(2+)]i和NE含量影响的实验研究

Experimental Studies on the Effection of 12 Hours Electro-acupuncture in [Ca～(2+)]i and NE Level of CCI rats

目的:对电针12h对颅脑损伤大鼠脑组织[Ca2+]i和NE含量的影响进行实验研究。方法:采用激光共聚焦显微镜的动态扫描功能和荧光分子探针技术,从针灸血清学和细胞学角度,对针刺通过颅脑损伤大鼠血清影响SY5Y细胞[Ca2+]i进行研究;采用高效液相色谱法研究电针对颅脑损伤大鼠脑组织NE含量的影响。结果:各组血清孕育12h后SY5Y细胞[Ca2+]i相比较显示,模型对照组的荧光强度明显高于空白对照组(P﹤0.01),针刺治疗组的荧光强度明显低于模型对照组(P﹤0.05);各组大鼠脑组织NE含量相比较,模型对照组显著高于空白对照组(P﹤0.01),针刺治疗组明显低于模型对照组(P﹤0.05)。结论:电针12h可减慢颅脑损伤大鼠脑组织[Ca2+]i和NE含量的上升趋势,提示电针可能是通过抑制脑皮质内大量NE聚集,降低皮质神经元的兴奋性,从而抑制细胞内Ca2+滞留和超载,减轻脑水肿,对脑组织起到保护作用。

Objective：To study on the effection of 12 hours electro-acupuncture in [Ca2＋ ]i and NE level of craniocerebral injury rats. Methods： From the ways of serology and cytology,Adopted Dynamic confocal laser scanning microscope and Fluorescent molecular probe techniques,to study on the influence of acupuncture in SY5Y cells[Ca2 ＋ ]i by the serum of CCI rats. To study the influence of electro-acupuncture in NE level of CCI brain tissue by high performance liquid chromatography. Results： Gestated by the serum of CCI rats,intercomparsion shows that SY5Y cells Fluorescent strength of the model group is higher than the normal group＇s（P ﹤ 0. 01）,and the acupuncture group＇s is lower than the model group＇s but higher than the normal group＇s（P ﹤ 0. 05）. Intercomparsion shows that NE levels of the model group is higher than the normal group＇s（P ﹤ 0. 01）,and the acupuncture group＇s is lower than the model group＇s but higher than the normal group＇s（P ﹤ 0. 05）. Conclusion： 12h electro-acupuncture on brain injury rats could slow down the upward trend of the [Ca2 ＋ ]i and NE level in rats brain tissue,suggesting that electro-acupuncture might inhibit NE accumulation in the brain cortex,reduce the excitability of cortical neurons,to inhibit retention and overload of intracellular Ca2 ＋ ,reduce brain edema and protect the brain tissue.