摘要
通过RT-PCR从人的脂肪组织中克隆了人OB基因,并将其在原核生物E.coli BL21(DE3)中重组表达,采用SDS-PAGE法检测表达情况,鉴定表达产物。结果表明,人瘦素在大肠杆菌中表达量占总蛋白的20%左右,为瘦素的进一步研究奠定了基础。
In this study, OB gene was cloned by RT-PCR from fat tissue of human and expressed recombinantly in E.cofi BL21(DE3) which was prokaryotic organism. The results showed that Leptin in E.cofi (DE3) was about 20% of total protein by SDS-PAGE. It lays a foundation for the study on Leptin further.
出处
《东北农业大学学报》
CAS
CSCD
北大核心
2010年第4期67-70,共4页
Journal of Northeast Agricultural University
基金
国家自然科学基金面上项目(30871431)
关键词
瘦素
基因克隆
原核表达
Leptin
gene cloning
prokaryotic expression