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人痩素基因的克隆及原核表达 被引量:2

Cloning and expression in prokaryotic organism of human leptin gene
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摘要 通过RT-PCR从人的脂肪组织中克隆了人OB基因,并将其在原核生物E.coli BL21(DE3)中重组表达,采用SDS-PAGE法检测表达情况,鉴定表达产物。结果表明,人瘦素在大肠杆菌中表达量占总蛋白的20%左右,为瘦素的进一步研究奠定了基础。 In this study, OB gene was cloned by RT-PCR from fat tissue of human and expressed recombinantly in E.cofi BL21(DE3) which was prokaryotic organism. The results showed that Leptin in E.cofi (DE3) was about 20% of total protein by SDS-PAGE. It lays a foundation for the study on Leptin further.
出处 《东北农业大学学报》 CAS CSCD 北大核心 2010年第4期67-70,共4页 Journal of Northeast Agricultural University
基金 国家自然科学基金面上项目(30871431)
关键词 瘦素 基因克隆 原核表达 Leptin gene cloning prokaryotic expression
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