小叶锦鸡儿种子中的生物碱及其细胞毒活性

Anticancer and phytochemical properties of alkaloids from Caragana microphylla

目的鉴定小叶锦鸡儿种子中的生物碱类成分,研究其抗癌活性。方法采用色谱和光谱法对小叶锦鸡儿种子中所含有的总生物碱进行分离并作结构鉴定;对小叶锦鸡儿种子的总生物碱及分离得到的5个生物碱单体分别进行细胞毒活性筛选。结果从总生物碱中共分离得到了5个原小檗碱型生物碱,分别为去氢白莲叶碱、黄连碱、表小檗碱、脱氢卡维丁以及刻叶紫堇明碱,它们对Saos-2,A375,Hela细胞株均表现出明显的细胞毒活性。结论5个生物碱单体均为首次从锦鸡儿属植物中分离得到,提示小叶锦鸡儿种子提取物具有抗肿瘤的药用价值。

Objective To identify the composition of the total alkaloids of the seeds of Caragana microphylla,a native plant from the North west of China which is used in traditional Mongolian medicines.Methods The alkaloids was separated and identified by chromatographic methods and spectral analysis.The anticancer activities of the total alkaloids and the isolated protoberberine alkaloids were tested respectively on Saos-2,A375 and Hela cell lines.Results Five protoberberine alkaloids including dehydrothalictrifoline,coptisine,epiberberine,dehydroapocavidine and corysamine were isolated.The totel alkaloids and the 5 alkaloids were found to be very effective against three tumor cell lines.Conclusions The 5 protoberberine alkaloids were isolated for the first time from Caragana genus.Caragana microphylla seed extracts may play a role as an hopefulanticancer therapeutic agent.