雾化吸入利多卡因对哮喘大鼠肺组织水通道蛋白5含量及白细胞介素-13表达的影响

Effect of aerosolized lidocaine inhalation on expression 是 of aquaporin 5 and IL-13 in asthma rats lung

目的观察雾化吸入利多卡因对大鼠支气管哮喘模型肺组织中水通道蛋白5(AQP5)表达及血清中白细胞介素-13(IL-13)浓度的影响。方法 Wistar大鼠32只,随机均分为四组:哮喘组(A组)、地塞米松组(D组)、利多卡因组(L组)和对照组(C组)。A组用鸡卵清蛋白辅以氢氧化铝为佐剂注射致敏,2周后雾化吸入鸡卵清蛋白诱发哮喘。D组、L组大鼠用同样方法致敏,但在激发前分别雾化吸入0.02%地塞米松和0.04%利多卡因20 min。C组用生理盐水代替鸡卵清蛋白进行注射和吸入。末次雾化吸入后24 h内采集静脉血,测定血清中IL-13的浓度;取肺组织,计算湿干重比(W/D),观察AQP5表达及肺组织病理学结果。结果与C组相比,A组肺组织AQP5表达降低,血清IL-13浓度及W/D增高(P<0.05);与A组相比,D、L组肺组织AQP5表达增高,血清中IL-13的浓度及W/D降低(P<0.05)。A组肺组织呈支气管壁增厚、炎性细胞浸润表现,D、L组病理损伤程度减轻。结论利多卡因雾化吸入可通过增加肺组织AQP5的表达,降低血清IL-13的浓度,减轻致敏原所激发的哮喘大鼠气道炎症。

Objective To observe the effects of aerosolized lidocaine inhalation on expressions of aquaporin-5（AQP5） and interleukin-13（IL13） in the lungs of asthma rat model. Methods Thirty- two Wistar rats were randomly assigned to four groups with 8 rats each. The rats in groups of A, D and L were sensitized by injection of ovalhumin（OA） together with aluminum hydroxide as adjuvants, followed by aerosolized OA challenge two weeks later. The rats of group D were exposed to 0.02% aerosol of dexamethasone and those in group L exposed to 0.04% aerosol of lidocaine for 20 min before the sensitization. Group C was not sensitized as the control. Venous blood was collected for detecting IL-13 at 24 hours after the last challenge and the lungs were removed for microscopic examination and determination of W/D. The expression of AQP5 was detected immunohistochemically. Results Compared with group C, the expression of AQP5 in the lungs in group A was significantly lower, but IL-13 and W/D lung weight radio were significantly higher. Compared to group A, the expression of AQP5 in the lungs in group D was significantly higher, but IL-13 and W/D of lung weight radio were significantly lower. The bronchial wall was significantly thicked with inflammatory cell infiltration in group A, which was lighter in groups of D and L. Conclusion The airway inflammation resulting from aeroallergen challenge could be reduced by aerosolized lidocaine inhalation in the asthma model of Wistar rats, which may be related to the decreases in the expressions of AQP5 in the lungs and IL-13 in serum.