摘要
目的:筛选黄连解毒汤中与治疗老年性疾病有关的有效成分。方法:运用紫外分光光度法,建立体外抗氧化模型和抑制乙酰胆碱酯酶(AchE)活性实验模型,分析比较了黄连解毒汤全方、单味药及其主要成分的抗氧化作用和对乙酰胆碱酯酶的抑制作用。结果:复方黄连解毒汤的抗氧化活性比黄连、黄柏和栀子单味药强;黄芩及其主要成分黄芩苷(IC50=12.06μg·mL-1)具有较强的抗氧化活性;与对照品毒扁豆碱和加兰他敏比较,小檗碱(IC50=9.52μg·mL-1)对乙酰胆碱酯酶的抑制活性较高,其次是黄连(IC50=18.89μg·mL-1)水提物,而黄芩和栀子抑制活性不明显。结论:黄连解毒汤具有抗氧化和抑制乙酰胆碱酯酶活性作用,其中具有多羟基的黄酮类是抗氧化活性的主要成分;而生物碱类则是抑制乙酰胆碱酯酶的主要成分。
Objective: To investigate and screen the active compounds of Huanglianjiedu decoction(HLJD) for senile dementia. Method: Inhibition on acetylcholinesterase and antioxidant activity by HLJD was studied with ultraviolet spectrometry. The single herbs and the major compounds of HLJD were also determined and compared by a rapid and effective acetylcholinesterase inhibition assay and antioxidant assay. Result: The formula HLJD had a better antioxidant activity than single herbs except for Radix Scutellaria which is rich in polyphenolic compounds. The most active conpounds was baicalin ( IC50 = 12. 06 μg·mL -1 ). The results also indicate that the most active herbs and compounds for AChE inhibition were Coptidis Rhizoma (IC50 = 18. 89 μg·mL -1) and berberine ( IC50 = 9. 52 μg·mL -1); Radix Scutellariae,Fructus Gardenia as well as the major compounds of them showed no or lower inhibitory activity. Conclusion: It can be concluded that alkaloid compounds in HLJD contribute to the inhibitory activity for acetylcholinesterase and flavanoids compounds contribute to the antioxidant activity.
出处
《中国实验方剂学杂志》
CAS
北大核心
2010年第5期61-64,共4页
Chinese Journal of Experimental Traditional Medical Formulae