白附子炮制后新增成分的分离和结构鉴定

Isolation and Identification of the Newly Added Chemical Constituent from Processed Rhizoma Typhonii

目的分离和鉴定白附子炮制后新增化学成分。方法采用高效液相色谱(HPLC)法确定提取溶剂,用硅胶柱和制备高效液相分离纯化,硅胶G薄层板和HPLC检识,通过理化性质和光谱数据确定化合物结构。结果新产生的化学成分为5-羟甲基糠醛(Ⅰ),还分离到了其结构类似物质双[5-甲酰基-糠基]-醚(Ⅱ)。结论首次从制白附子中分离得到5-羟甲基糠醛和双[5-甲酰基-糠基]-醚,为中医临床炮制用药提供依据。

Objective To study the newly added chemical constituent from processed Rhizoma Typhonii.Methods The extraction solvent was determined by HPLC.The constituents were isolated and purified by column chromatography and preparative HPLC method and determined by TLC and HPLC.The structures were elucidated based on chemical and spectral analysis.Results Two compounds were isolated from the chloroform extracts.Their structures were determined as 5-hydroxymethyl-2-furaldehyde （Ⅰ） and bis（5-formylfurfuryl）ether（Ⅱ）.Conclusion 5-hydroxymethyl-2-furaldehyde and bis（5-formylfurfuryl）ether were isolated from processed Rhizoma Typhonii for the first time.