摘要
目的比较白芍不同种质的芍药苷含量差异。方法 :采用高效液相色谱法,色谱条件:LichroCART RP-C18(4×250mm,5μm);流动相:乙腈-0.1%磷酸溶液(14:86);流速:1mL/min;检测波长:230nm;柱温:30℃;进样量:10μL。结果白芍不同种质芍药苷含量存在较大差异,白芍地下各部分芍药苷的含量差异呈现根茎(芍头)>须根>主根。结论芍药苷含量差异的产生主要来源于种质的不同。
Objective To compare the content of paeoniflorin from different germplasms Paeonia lactifolra.Methods HPLC method was used on LichroCART RP-C18 column（4 mm×250 mm,5 μm）with HCN- 0.1% phosphoric acid（V：V=14：86） as mobile phase,the flow rate was 1.0 mL/min,the detection wavelength was at 230 nm and column temperature was 30 ℃.Results There was great difference that Paeoniflorin in different germplasms was quite different.Determinated of peoniflorin in different parts of Radix Paeonia Alba reveals was in terms of content of peoniflorin： rhizomafi brous rootaxial root.Conclusion The difference of peoniflorin content was mainly from germplasms source of Paeonia lactiflora .
出处
《现代中药研究与实践》
CAS
2010年第4期75-78,共4页
Research and Practice on Chinese Medicines
基金
国家科技支撑计划(2006BAI06A12-07)
关键词
白芍
种质
芍药苷
Paeonia lactiflora
germplasm
paeoniflorin
