人晚期糖基化产物受体在前列腺癌和正常前列腺组织的表达差异

Differential expressions of the receptor for advanced glycation end products in prostate cancer and normal prostate

目的:研究人晚期糖基化产物受体(receptor for advanced glycation end product,RAGE)在前列腺癌和正常前列腺组织中的表达差异,为进一步研究RAGE在前列腺癌发病机制中的作用奠定基础。方法:以同一患者的前列腺癌组织和正常前列腺组织配对作比较,采用免疫组化染色法、免疫印迹法、实时荧光定量PCR等方法分别从组织水平、蛋白质水平、mRNA水平检测10例患者前列腺癌和正常前列腺组织中RAGE的表达。结果:免疫组化结果显示RAGE在前列腺癌组织中的表达水平明显高于正常前列腺组织,Western印迹检测发现前列腺癌组织RAGE的表达量是正常前列腺组织的2.13倍(Р<0.05),荧光定量PCR检测前列腺癌中RAGE的mRNA表达水平是正常前列腺组织的4.2倍(Р<0.05)。结论:RAGE在前列腺癌的发生和发展过程中可能起重要作用。

Objective：To study the differential expressions of the receptor for advanced glycation end products （RAGE） in the tissues of prostate cancer and normal prostate,and to find the role of RAGE in the pathogenesis of prostate cancer.Methods：We collected the tissue of prostate cancer and that of normal prostate from the same patient,and compared the differential expressions of RAGE at the tissue,protein and mRNA levels between prostate cancer and normal prostate tissues of 10 patients by immunohistochemisty,Western blot and real-time quantitative PCR.Results：Immunohistochemistry exhibited a significantly higher expression of RAGE in the prostate cancer tissue than in the normal prostate tissue;Western blot showed that the RAGE protein expression was 2.13 times higher in the former than in the latter （Р 0.05）;and real-time quantitative PCR revealed the RAGE mRNA expression of the former to be 4.2 times that of the latter （Р 0.05）.Conclusion：RAGE may play an important role in the pathogenesis and progression of prostate cancer.