摘要
目的:研究黄芪甲苷对帕金森病(PD)体外、体内模型的神经保护作用。方法:MPP+诱导PC12细胞损伤复制体外PD模型,1-甲基-4-苯基-1,2,3,6-四氢吡啶(MPTP)复制PD小鼠模型。采用MTT法测定黄芪甲苷对MPP+诱导的PC12细胞的存活率及培养液中乳酸脱氢酶(LDH)和丙二醛(MDA)的影响,以及对模型小鼠自发活动及纹状体多巴胺(DA)及其高香草酸(HVA)含量的影响。结果:25、50、100μmol·L-1黄芪甲苷可呈浓度依赖性抑制MPP+诱导的PC12细胞存活率降低,并显著降低培养上清液中LDH和MDA的含量;10、20、40mg·kg-1黄芪甲苷可显著增加模型小鼠自发活动计数值,并显著降低纹状体DA及HVA的含量。结论:黄芪甲苷对PD体外、体内模型均有显著的神经保护作用。
OBJECTIVE: To study the protective effect of astragaloside on nerve of in vitro and in vivo model of Parkinson disease (PD).METHODS: MPP+ induced PC12 cell injury to establish in vitro PD model.PD mice model was induced by MPTP.MTT assay was used to determine the effect of astragaloside on survival rate of PC12 cell induced by MPP+ and the content of LDH and MDA.The effect of astragaloside on spontaneous behavior and the content of striatal DA and HVA were also detected.RESULTS: 25 μmol·L-1,50 μmol·L-1,100 μmol·L-1 dose of astragaloside inhibited the decrease of survival rate of PC12 cell induced by MPP+ in dose dependent manner.10 μmol·L-1,20 μmol·L-1,40 μmol·L-1 dose of astragaloside can obviously enhanced spontaneous behavior of model mice,and reduced the content of striatal DA and HVA.CONCLUSION: Astragaloside can protect nerve of in vivo PD and in vitro PD model.
出处
《中国药房》
CAS
CSCD
北大核心
2010年第23期2132-2134,共3页
China Pharmacy