摘要
目的观察针灸预刺激对老年性痴呆模型大鼠神经元凋亡相关蛋白Bcl-2、NF-κB的影响。方法对治疗组大鼠分别予电针、艾灸和电针加艾灸百会和肾俞穴8周后,在大鼠双侧海马区注射Aβ1-40,建立AD大鼠模型,通过Morris水迷宫检测行为学,免疫组化检测大鼠海马组织Bcl-2、NF-κB表达。结果水迷宫检测行为学发现模型组大鼠学习记忆能力有明显损害,与正常组、假手术组有显著差异(P<0.01);免疫组化结果显示Aβ1-40能使大鼠海马NF-κBP65蛋白表达明显增加,Bcl-2蛋白表达相应减少(P<0.01);而针灸预刺激各组NF-κBP65蛋白的表达明显减少,Bcl-2蛋白表达相应增加(P<0.01);各治疗组检测结果显示预针灸组效果最佳(P<0.05),预电针组和预艾灸组无明显差异(P>0.05)。结论针灸预刺激可通过抑制NF-κB表达,增加Bcl-2的表达,保护神经细胞,维持正常学习记忆能力,延缓AD进程。
Objective To explore the mechanism of acupuncture on Alzheimer's disease (AD) prevention and treatment by observing the influences of pre-acupuncture stimulation on neuronal apoptosis-related protein Bcl-2,NF-κB in AD rat models.Methods For the treatment group,rats were treated with electro-acupuncture,moxibustion and electro-acupuncture plus moxibustion at Baihui (DU 20) and Shenshu (BL 23) points for eight weeks,after which AD rat model was established via bilateral injection of Aβ1-40 in hippocampus.Rat praxiology was evaluated through the Morris watermaze test.Immunohistochemistry was applied for detecting rat hippocampus Bcl-2,NF-κB expression.Results The Morris water maze test showed obvious impairment in rat learning and memorizing ability,with significant difference observed in comparison with the normal group and sham operation group (P 0.01).Immunohistochemistry results showed that Aβ1-40 increased hippocampus NF-κB P65 protein expression significantly,with reduced Bcl-2 protein expression correspondently (P 0.01).NF-κB P65 protein expression in each pre-acupuncture stimulation groups was significantly reduced,Bcl-2 protein expression was in a corresponding increase (P 0.01).Each treatment groups test results showed the best effect existed in the pre-acupuncture group (P 0.05),pre-electro-acupuncture group and the pre-moxibustion group were of no significant difference (P 0.05).Conclusions Acupuncture pre-stimulation slows down AD progression by protecting neuronal cells and maintaining normal ability of learning and memorizing via inhibiting NF-κB expression and promoting expression of Bcl-2.
出处
《湖北中医学院学报》
2010年第3期16-18,共3页
Journal of Hubei College of Traditional Chinese Medicine
基金
国家自然科学基金(30772837)
关键词
针灸预刺激
AD
BCL-2
NF-ΚB
Pre-stimulation of acupuncture and moxibustion
Alzheimer's disease (AD)
Bcl-2
NF-κB