秋冬季节对健康大鼠肺组织表面活性蛋白A和白细胞介素6表达的影响

Effect of autumn and winter seasons on expression of surfactant associated protein A and interleukin-6 in healthy rat lung tissues

背景:近年来研究证实,季节气候的变化和肺表面活性物质蛋白A及白细胞介素6在肺脏免疫防御功能调节方面均起到很重要的作用,但是季节气候产生影响的机制目前还不清楚。目的:观察秋冬季大鼠肺组织表面活性蛋白A和白细胞介素6的表达,认识季节和气候变化影响大鼠肺脏非特异性免疫的作用机制,为呼吸系统季节性发病的病理生理机制的认识提供实验依据。方法:分别于立秋、秋分、立冬、冬至节气前14d购入雄性SD实验大鼠,并分为相应组别。自由摄取水及正常饲料和室温饲养,接受自然光照。到相应节气,于当日下午6时将动物断头处死,取出全肺组织作表面活性蛋白AmRNA和白细胞介素6mRNA检测。结果与结论:与立秋、秋分、冬至组大鼠相比,立冬大鼠肺组织表面活性蛋白A和白细胞介素6均表达量较低,提示肺部免疫防御功能存在秋季高冬季低的季节节律,而表面活性蛋白A和白细胞介素6正是免疫防疫功能的物质基础。

BACKGROUND： Previous study has confirmed that seasonal variation and surfactant associated protein A （SP-A） and interleukin-6 （IL-6） exhibit a significant role in lung immune and defense function. However, the effect mechanism of them remains unclear. OBJECTIVE： By observing the changes of SP-A and IL-6 of male rats in autumn and winter, to explore the effects of seasonal changes on the nonspecific immunity of lung of normal rats, and to provide new ideas for the experimental basis to the cognition of pathology and physiology mechanism for seasonal attack of respiratory system diseases. METHODS： Male SD rats were purchased from each seasons and divided into groups in chronological order. All rats were housed with normal forage and drank freely under room temperature. Then the rats were sacrificed by decapitation before 18 o’clock at corresponding solar term, and the lung tissues were analyzed by RT-PCR. RESULTS AND CONCLUSION： Compared to the other groups, the expression of SP-A and IL-6 in the Winter Begins group were smaller, which demonstrated that immune function of lung was existed the seasonal rhythm of lower in winter and higher in autumn. The SP-A and IL-6 are main material basis of immune function.