期刊文献+

水疱性口炎病毒单克隆抗体的制备与生物学特性鉴定 被引量:5

Production and Biologic Characterization of Monoclonal Antibodies Against Vesicular Stomatitis Virus
下载PDF
导出
摘要 用纯化的水疱性口炎毒(VSV)免疫接种Balb/c小鼠,取免疫接种的小鼠脾细胞与SP2/0骨髓瘤细胞进行细胞融合,经间接ELISA筛选,有限稀释法克隆,获得4株稳定分泌抗VSV特异性单克隆抗体的杂交瘤细胞株(5B1、8H1、9B9和11E11)。4株单克隆抗体均能与VSV反应,且不与其他病毒及BHK细胞反应,表明4株单克隆抗体特异性良好。4株单克隆抗体腹水ELISA效价分别为1∶512 000、1∶256000、1∶256 000、1∶1 024 000。本试验为研究VSV生物学特性和建立VSV免疫学检测方法奠定了基础。 Monoclonal antibodies(McAbs) 5B1,8H1,9B9 and 11E11 against vesicular stomatitis virus(VSV) were produced by fusing SP2/0 myeloma cells with spleen cells of Balb/c mice immunized with purified VSV.The ELISA titers of 4 McAbs in mouse ascites were 1∶512 000,1∶256 000,1∶256 000 and 1∶1 024 000,respectively.These four McAbs could specifically react with VSV but not cross react with other viruses and BHK when tested using indirect ELISA based on purified VSV,BHK and other viruses.The McAbs are helpful tools for studying the biologic characterization of VSV and developing diagnostic techniques of VSV.
出处 《动物医学进展》 CSCD 北大核心 2010年第7期6-10,共5页 Progress In Veterinary Medicine
基金 国家863计划项目(2006AA10Z445)
关键词 水疱性口炎病毒 单克隆抗体 生物学特性 Vesicular stomatitis virus monoclonal antibodies biological characteristic
  • 相关文献

参考文献8

二级参考文献33

  • 1杨书豪,王伟,景怀奇,田素娟,刘奇,徐建国,姚楚铮,徐海燕.抗出血性大肠杆菌Ο157∶H7单克隆抗体的制备及鉴定[J].中国人兽共患病杂志,2004,20(11):963-965. 被引量:5
  • 2陈萍,刘军,祝令伟,孙洋,郭学军,齐翀,冯书章,郑明光.出血性大肠杆菌O157 eae-stx1/2B融合基因的构建和表达[J].中国兽医学报,2005,25(3):270-272. 被引量:3
  • 3梁荣,伊岚,李健强.小鼠IgG类单克隆抗体三种纯化方法的比较[J].细胞与分子免疫学杂志,1996,12(1):55-58. 被引量:13
  • 4梅明珠,严亚贤,陆承平.VT2-B亚单位的重组表达及单克隆抗体的制备[J].微生物学通报,2007,34(3):475-478. 被引量:1
  • 5MIKHAIL D, SCOTT F, CAROL H, et al. Classification and nomen culture of viruses[A]//FAUQUET C M, MAYO M A,MANILOFF J,et al. Virus Taxonomy: Ⅷ th Report of the International Committee on Taxonomy of Viruses(Hardcover) [C].New York, USA : ICTVdB Management, 2002 : 652 -686.
  • 6CHASE C C, BRAUN L J, LESLIE-STEEN P, et al. Bovine viral diarrhea virus multiorgan infection in two white-tailed deer in southeastern South Dakota[J].Wildl Dis,2008,44(3):753- 759.
  • 7SEMRAU A, WIBBELT G, HILBE M, et al. Availability of oral swab sample for the detection of bovine viral diarrhea virus(BVDV) gene from the cattle persistently infected with BVDV[J]. Vet Res ,2008,56(1) :3-8.
  • 8VAN RIJN P A. A common neutralizing epitope on envelope glyeoprotein E2 of different pestiviruses: Implications for improvement of vaccines and diagnostics for classical swine fever (CSF) ? [J].Vet Microbiol, 2007,125(1/2) : 150-156.
  • 9HERMAN N, SCHAGG E R, BAESE H J, et al. Tricinesodium dodecyl sulfate polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 ku [J]. Anal Biockem , 1987,166(5) :368-369.
  • 10PETRIC M, YOI.KEN R H, DUBOVI E J, et al. Baculovirus expression of pestivirus non-structural protein[J].Gen Virol, 1992,73(2):1867-1871.

共引文献11

同被引文献45

  • 1郑增忍,王海霞,龚振华,张彦明.水疱性口炎研究进展[J].中国兽医杂志,2005,41(7):32-35. 被引量:8
  • 2李慧昕,王君伟.牛病毒性腹泻病毒生物型转化分子机制的研究进展[J].中国兽医科技,2005,35(8):657-660. 被引量:14
  • 3秦晓光,尹荣兰,沈国顺.小反刍兽疫的研究进展[J].现代畜牧兽医,2006(1):31-33. 被引量:18
  • 4刘玉洪,花群义,徐自忠,杨云庆,周晓黎,董俊,尹尚莲,许靖逸,高洪.小反刍兽疫病毒H糖蛋白基因原核表达载体的构建及表达[J].中国兽医科学,2006,36(9):692-695. 被引量:11
  • 5萨姆布鲁克J 拉塞尔DW 黄培堂译.分子克隆实验指南(第3版)[M].北京:科学出版社,2002..
  • 6王世若.现代动物免疫学[M].吉林:吉林科技出版社,2001.
  • 7Ridpath J F, Bolin S R, Dubovi E J. Segregation of bovine viral diarrhea virus into genotypes [ J]. Virology, 1994, 205 (1) 66-74.
  • 8Ridpath J F, Neill J D, Frey M, et al. Phylogenetic, antigenic and clinical characterization of type 2 BVDV from North America [J]. Vet Microbiol,2000,77(1-2) : 145-155.
  • 9Makoschey B, Sonnemans D, Bielsa J M, et al. Evaluation of the induction of NS3 specific BVDV antibodies using a commercial in- activated BVDV vaccine in immunization and challenge trials [J]. Vaccine, 2007, 25(32): 6140-6145.
  • 10Bhatia S, Sood R, Mishra N, et al. Development and evaluation of a MAb based competitive-ELISA using helicase domain of NS3 protein for sero-diagnosis of bovine viral diarrhea in cattle and buffaloes [J]. Res Vet Sci, 2008, 85(1):39-45.

引证文献5

二级引证文献6

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部