腹腔注射环磷酰胺对小鼠精子凋亡的影响

The effects of cyclophosphamide peritoneal injection to mice spermatozoa

目的观察腹腔注射环磷酰胺对小鼠精液常规和精子凋亡的影响。方法将20只雄性昆明小鼠随机分为对照组和实验组,按60 mg/kg体重给对照组小鼠腹腔注射NS,60 mg/kg体重给实验组小鼠腹腔注射环磷酰胺,每天1次,连续5 d,常规饲养观察34 d,然后对小鼠睾丸进行病理切片观察病理形态变化,对小鼠精子进行精液常规分析,对精子行FITC-AV/PI染色后用流式细胞仪检测精子凋亡率。结果实验组小鼠睾丸生精细胞、间质细胞和部分支持细胞明显变性,对照组与实验组精子密度分别为(5.20±1.34)×106/ml、(1.73±0.03)×106/ml,精子活力分别为(14.49±0.30)%、(6.64±1.88)%,精子成活率分别为(68.39±15.13)%、(39.96±4.89)%,精子凋亡率分别为8.38±0.45、14.84±1.22,两组比较有显著差异(P<0.05)。结论腹腔注射环磷酰胺可使小鼠睾丸生精功能降低,精子凋亡增加,造成小鼠少弱精症。

Objective To observe sperm count,motility,survival rate and apoptosis after peritoneal injected cyclophosphamide to mice.Methods 20 KUNMING male mice were randomly taken to control group and model group.During the first 5 days,control group were peritoneal injected NS with 60mg/kg weight once a day,while model group injected Cyclophosphamide,and then all mice were normally breed for 34 days.After that,it was analyzed that morphologic change of pathological section of mice testis,the sperm count,motility and survival rate were measured,and the apoptosis of sperm,dye with FITC-AV/PI,was measured by flow cytometer.Results The spermatogenic cells,stromal cells and part sertoli cells of model group were denaturation,and the sperm count,motility,survival rate and apoptosis rate of control group was（5.20±1.34）×106/ml,（14.49±0.30）%,（68.39±15.13）% and 8.38±0.45,while model group（1.73±0.03）×106/ml,（6.64±1.88）%,（39.96±4.89）% and 14.84±1.22（P0.05）.Conclusion After cyclophosphamide peritoneal injection,the gonepoiesis of mice decreased,the apoptosis of sperm increased.