买提布合艾菲提蒙方醇提物对高脂血症大鼠血脂水平的影响

Effect of Ethanol Extract from Matbuhi Aftimun on Blood Lipids Level in Rat Hyperlipidemia Model

目的探讨维吾尔医经典方剂买提布合艾菲提蒙方醇提物的毒性、降血脂作用及其机制。方法(1)用简化机率单位法测定小鼠买提布合艾菲提蒙方醇提物半数致死量或最大耐受量。(2)高脂乳剂灌胃SD大鼠形成高脂血症动物模型,观察买提布合艾菲提蒙方醇提物不同浓度对血清总胆固醇(TC)、低密度脂蛋白-胆固醇(LDL-C)、高密度脂蛋白-胆固醇(HDL-C)、甘油三酯(TG)及肝组织匀浆超氧化物歧化酶(SOD)、丙二醛(MDA)、谷胱甘肽过氧化物酶(GSH-PX)、总脂酶[包括脂蛋白脂酶(LPL)及肝脂酶(HL)]水平的影响。结果(1)买提布合艾菲提蒙方醇提物小鼠最大耐受剂量为64 g(生药)/kg。(2)买提布合艾菲提蒙方醇提物(0.8、1.6、3.2 g/kg)能降低血清TC水平(P<0.05,P<0.01),对血清LDL-C、HDL-C、TG水平的影响无统计学意义(P>0.05)。同时,买提布合艾菲提蒙方醇提物(0.8、1.6、3.2 g/kg)升高肝匀浆MDA水平(P<0.01);对肝匀浆GSH-PX、SOD、总脂酶水平的影响无统计学意义(P>0.05)。结论买提布合艾菲提蒙方醇提物对小鼠毒性小,且对实验性高脂血症大鼠有降低血清TC水平的作用。

Objective To investigate the acute toxicity, lipid reducing effect and mechanism of action of ethanol extracts of Matbuhi Aftimun （ E-MA）, a classic prescription of Uighur medicine, on hyperlipidemia rat model. Methods The LD50 or maximum tolerance of rats to E-MA was determined by simplified probit method. Hyperlipidemia rat model was established in SD rats by feeding high lipid emulsion, then E-MA at different dosages （0.80 g/kg, 1.60 g/kg and 3.20 g/kg） was given orally to them. The effects of E-MA on model rats＇ serum lipids, including total cholesterol （TC）, low density lipoprotein-cholesterol （ LDL-C）, high density lipoprotein-cholesterol （HDL-C）, triglyceride （TG）, were observed. And its effects on malondialdehydec （MDA） content, superoxide dismutase （ SOD）, glutathione peroxidase （ GSH-PX）, total lipase, including lipoprotein lipase （LPL） and hepato-lipase （HL） activities in the liver homogenate were assayed. Results The maximum tolerance of rats to E-MA was 64 g （crude drug）/kg. Compared with the hyperlipidemia model rat, the blood TC level was lower （P 〈0.05 or P 〈 0.01 ）, and the MDA content in the liver homogenate was higher in model rat after E-MA treatment （at all the three specified dosages, P 〈0.01 ）, but no significant difference was found in comparisons of serum levels of LDL-C, HDL-C and TG （ P 〉 0. 05）, also on the levels of SOD, GSH-PX and total lipase in the liver homogenate （ P 〉0.05）. Conclusion E-MA shows a serum TC reducing effect on hyperlipidemia rat model with low toxicity in mice.