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rRTA:DS27,ricin:DS27两种免疫毒素的初步比较 被引量:3

Preliminary Comparison between rRTA : DS27 and ricin: DS27 as Immunotoxins
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摘要 目的:用重组的蓖麻毒素A链与抗CD5的单克隆抗体DS27交联为免疫毒素rTRA:DS27,与完整蓖麻毒素制备的ricin:DS27进行对比研究.方法:分析它们对靶细胞的特异性杀伤,对骨髓造血细胞增殖的影响,内化情况,以及NH_4cl对rRTA:DS27毒性的增强作用.结果:rRTA:DS27具有更好的导向特异性,对骨髓造血功能影响较小,NH_4cl能明显增强rRTA:DS27的毒性.结论:rTRA:DS27免疫毒素具有更多的优点. Objective: Immunotoxin rRTA:DS27, which was prepared by conjugating DS27 with recombinanl ricin a chain, was compared with ricin:DS27 as immunotoxins. Methods: System analysis were performed regard to their cell-specific cytotoxicity, inhibition on the proliferation of hemoapeutic potential cells, immunogold-labelled intracellular routing and effect of NH_(4)Cl on the cytotoxicity. Results: Results showed that rRTA: DS27 got a more specific cytotoxicity and a weaker inhibition on the proliferation of hemoapeutic potential cells than ricin:DS27, NH_(4)Cl could obsolutelyy enhence the cytotoxicity of rRTA:DS27. Conclusion: rRTA:DS27 had more advantages than ricin: DS27 as immunotoxins.
出处 《中国肿瘤生物治疗杂志》 CAS CSCD 1999年第1期4-7,共4页 Chinese Journal of Cancer Biotherapy
基金 国家"863"生物技术项目(102-09-01-03)资助
关键词 蓖麻毒素A链 免疫毒素 导向药物 recombinant ricin toxin a chain immunotoxin targeting drug
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